ArticleMolecular biotechnology2013
Construction and application of a food-grade expression system for Lactococcus lactis.
Article in Molecular biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 27 citations in OpenAlex.
- Development of a lactose-complemented antibiotic-free host-vector platform for stable enzyme expression in Limosilactobacillus fermentum 217 - 82.World journal of microbiology & biotechnology · 2026Article
- Promoters for the expression of food-grade selectable markers in lactic acid bacteria and bifidobacteria.Applied microbiology and biotechnology · 2022Article
- Plasmid-Based Gene Expression Systems for Lactic Acid Bacteria: A Review.Microorganisms · 2022Review
- Protective effects of a food-grade recombinant Lactobacillus plantarum with surface displayed AMA1 and EtMIC2 proteins of Eimeria tenella in broiler chickens.Microbial cell factories · 2020Article
- A new approach to obtain pure and active proteins from Lactococcus lactis protein aggregates.Scientific reports · 2018Article
- PilVax - a novel peptide delivery platform for the development of mucosal vaccines.Scientific reports · 2018Article
- Fluorescent Lactic Acid Bacteria and Bifidobacteria as Vehicles of DNA Microbial Biosensors.International journal of molecular sciences · 2017Article
- Development of a counterselectable seamless mutagenesis system in lactic acid bacteria.Microbial cell factories · 2017Article
- Bacterial mimetics of endocrine secretory granules as immobilized in vivo depots for functional protein drugs.Scientific reports · 2016Article
- Nisin Z Production by Lactococcus lactis subsp. cremoris WA2-67 of Aquatic Origin as a Defense Mechanism to Protect Rainbow Trout (Oncorhynchus mykiss, Walbaum) Against Lactococcus garvieae.Marine biotechnology (New York, N.Y.) · 2015Article
- Plasmids from Food Lactic Acid Bacteria: Diversity, Similarity, and New Developments.International journal of molecular sciences · 2015Review
- A bacteriocin gene cluster able to enhance plasmid maintenance in Lactococcus lactis.Microbial cell factories · 2014Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A food-grade host/vector expression system for Lactococcus lactis was constructed using alanine racemase gene (alr) as the complementation marker. We obtained an alanine racemase auxotrophic mutant L. lactis NZ9000Δalr by double-crossover recombination using temperature-sensitive integration plasmid pG(+)host9 and a food-grade vector pALR with entirely lactococcal DNA elements, including lactococcal replicon, nisin-inducible promoter PnisA and the alr gene from Lactobacillus casei BL23 as a complementation marker. By using the new food-grade host/vector system, the green fluorescent protein and capsid protein of porcine circovirus type II were successfully overexpressed under the nisin induction. These results indicate that this food-grade host/vector expression system has application potential as an excellent antigen delivery vehicle, and is also suitable for the use in the manufacture of ingredients for the food industry.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.