ArticleBMC microbiology2012
Development and validation of a real-time PCR assay for detection and quantification of Tuber magnatum in soil.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 34 citations in OpenAlex.
- Newly Designed Fluorescence In Situ Hybridization Probes Reveal Previously Unknown Endophytic Abilities of Tuber magnatum in Herbaceous Plants.Microbial ecology · 2025Article
- Advances in molecular genetics have increased knowledge of Tuber species' life cycle and population genetic structure, indicating ways to improve yield.Mycorrhiza · 2024Review
- Sustainable cultivation of the white truffle (Tuber magnatum) requires ecological understanding.Mycorrhiza · 2023Review
- Design and Validation of qPCR-Specific Primers for Quantification of the MarketedJournal of fungi (Basel, Switzerland) · 2022Article
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- First production of Italian white truffle (Tuber magnatum Pico) ascocarps in an orchard outside its natural range distribution in France.Mycorrhiza · 2021Article
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- Effect of earthworms on mycorrhization, root morphology and biomass of silver fir seedlings inoculated with black summer truffle (Tuber aestivum Vittad.).Scientific reports · 2021Article
- Quantification of DNA through the NanoDrop Spectrophotometer: Methodological Validation Using Standard Reference Material and Sprague Dawley Rat and Human DNA.International journal of analytical chemistry · 2020Article
- Soil temperature and hydric potential influences the monthly variations of soil Tuber aestivum DNA in a highly productive orchard.Scientific reports · 2019Article
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- Assessment of ectomycorrhizal fungal communities in the natural habitats of Tuber magnatum (Ascomycota, Pezizales).Mycorrhiza · 2013Article
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundTuber magnatum, the Italian white truffle, is the most sought-after edible ectomycorrhizal mushroom. Previous studies report the difficulties of detecting its mycorrhizas and the widespread presence of its mycelium in natural production areas, suggesting that the soil mycelium could be a good indicator to evaluate its presence in the soil. In this study a specific real-time PCR assay using TaqMan chemistry was developed to detect and quantify T. magnatum in soil. This technique was then applied to four natural T. magnatum truffières located in different regions of Italy to validate the method under different environmental conditions.
resultsThe primer/probe sets for the detection and quantification of T. magnatum were selected from the ITS rDNA regions. Their specificity was tested in silico and using qualitative PCR on DNA extracted from 25 different fungal species. The T. magnatum DNA concentration was different in the four experimental truffières and higher in the productive plots. T. magnatum mycelium was however also detected in most of the non-productive plots. Ascoma production during the three years of the study was correlated with the concentration of T. magnatum DNA.
conclusionsTaken together, these results suggest that the specific real-time PCR assay perfected in this study could be an useful tool to evaluate the presence and dynamics of this precious truffle in natural and cultivated truffières.
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