ArticleThe European journal of neuroscience2012
The development, distribution and density of the plasma membrane calcium ATPase 2 calcium pump in rat cochlear hair cells.
Article in The European journal of neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 53 citations in OpenAlex.
- Calmodulin controls spatial and temporal specificity of calcium-induced calcium release.PLoS computational biology · 2026Article
- CaProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Compensatory Interplay Between Clarin-1 and Clarin-2 Deafness-Associated Proteins Governs Phenotypic Variability in Hearing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- The RNA-binding protein TRIM71 is essential for hearing in humans and mice and times auditory sensory organ development.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- CaNature communications · 2025Article
- Hair cell apoptosis and deafness inProceedings of the National Academy of Sciences of the United States of America · 2025Article
- The Role of Calcium Signaling in Sensorineural Hearing Loss.International journal of medical sciences · 2025Review
- Jag1 represses Notch activation in lateral supporting cells and inhibits an outer hair cell fate in the medial cochlea.Development (Cambridge, England) · 2024Article
- SUB-immunogold-SEM reveals nanoscale distribution of submembranous epitopes.Nature communications · 2024Article
- SUB-Immunogold-SEM reveals nanoscale distribution of submembranous epitopes.Research square · 2024Article
- Regulation of cochlear hair cell function by intracellular calcium stores.Frontiers in cellular neuroscience · 2024Article
- Mitochondrial form and function in hair cells.Hearing research · 2023Review
- Clinical features and prognosis of pediatric idiopathic sudden sensorineural hearing loss: A bi-center retrospective study.Frontiers in neurology · 2023Article
- Oncomodulin (OCM) uniquely regulates calcium signaling in neonatal cochlear outer hair cells.Cell calcium · 2022Article
- Collapsin Response Mediator Protein 1 (CRMP1) Is Required for High-Frequency Hearing.The American journal of pathology · 2022Article
- Influence of T-Bar on Calcium Concentration Impacting Release Probability.Frontiers in computational neuroscience · 2022Article
- Synaptic Contributions to Cochlear Outer Hair Cell CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- Mitochondrial calcium uniporter is essential for hearing and hair cell preservation in congenic FVB/NJ mice.Scientific reports · 2021Article
- MET currents and otoacoustic emissions from mice with a detached tectorial membrane indicate the extracellular matrix regulates CaThe Journal of physiology · 2021Article
- Electron Microscopy Techniques for Investigating Structure and Composition of Hair-Cell Stereociliary Bundles.Frontiers in cell and developmental biology · 2021Article
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
Abstract
Calcium is tightly regulated in cochlear outer hair cells (OHCs). It enters mainly via mechanotransducer (MT) channels and is extruded by the plasma membrane calcium ATPase (PMCA)2 isoform of the PMCA, mutations in which cause hearing loss. To assess how pump expression matches the demands of Ca(2+) homeostasis, the distribution of PMCA2 at different cochlear locations during development was quantified using immunofluorescence and post-embedding immunogold labeling. The PMCA2 isoform was confined to stereociliary bundles, first appearing at the base of the cochlea around post-natal day (P)0 followed by the middle and then the apex by P3, and was unchanged after P8. The developmental appearance matched the maturation of the MT channels in rat OHCs. High-resolution immunogold labeling in adult rats showed that PMCA2 was distributed along the membranes of all three rows of OHC stereocilia at similar densities and at about a quarter of the density in inner hair cell stereocilia. The difference between OHCs and inner hair cells was similar to the ratio of their MT channel resting open probabilities. Gold particle counts revealed no difference in PMCA2 density between low- and high-frequency OHC bundles despite larger MT currents in high-frequency OHCs. The PMCA2 density in OHC stereocilia was determined in low- and high-frequency regions from calibration of immunogold particle counts as 2200/μm(2) from which an extrusion rate of ∼200 ions/s per pump was inferred. The limited ability of PMCA2 to extrude the Ca(2+) load through MT channels may constitute a major cause of OHC vulnerability and high-frequency hearing loss.
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