ReviewJournal of neurochemistry2012
Real-time imaging of plasma membrane deformations reveals pre-fusion membrane curvature changes and a role for dynamin in the regulation of fusion pore expansion.
Review in Journal of neurochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
What it found
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Who cites it
21 citing papers in PubMed.
- Requirement of Cholesterol for Calcium-Dependent Vesicle Fusion by Strengthening Synaptotagmin-1-Induced Membrane Bending.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Visualization of Partial Exocytotic Content Release and Chemical Transport into Nanovesicles in Cells.ACS nano · 2022Article
- Fluid markers of synapse degeneration in synucleinopathies.Journal of neural transmission (Vienna, Austria : 1996) · 2022Review
- Optimal Detection of Fusion Pore Dynamics Using Polarized Total Internal Reflection Fluorescence Microscopy.Frontiers in molecular biosciences · 2021Article
- Integrating Optical and Electrochemical Approaches to Assess the Actions of Dynamin at the Fusion Pore.Methods in molecular biology (Clifton, N.J.) · 2020Article
- Imaging Membrane Curvature inside a FcεRI-Centric Synapse in RBL-2H3 Cells Using TIRF Microscopy with Polarized Excitation.Journal of imaging · 2019Article
- Serial block-face scanning electron microscopy reveals neuronal-epithelial cell fusion in the mouse cornea.PloS one · 2019Article
- Membrane bending occurs at all stages of clathrin-coat assembly and defines endocytic dynamics.Nature communications · 2018Article
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- The Detection of Nanoscale Membrane Bending with Polarized Localization Microscopy.Biophysical journal · 2017Article
- Nanoscale Membrane Budding Induced by CTxB and Detected via Polarized Localization Microscopy.Biophysical journal · 2017Article
- Refractive Index Imaging of Cells with Variable-Angle Near-Total Internal Reflection (TIR) Microscopy.Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada · 2017Article
- The Calcineurin-Binding, Activity-Dependent Splice Variant Dynamin1xb Is Highly Enriched in Synapses in Various Regions of the Central Nervous System.Frontiers in molecular neuroscience · 2017Article
- Review
- Imaging plasma membrane deformations with pTIRFM.Journal of visualized experiments : JoVE · 2014Article
- Actin controls the vesicular fraction of dopamine released during extended kiss and run exocytosis.ACS chemical biology · 2014Article
- Dynamin-2 function and dysfunction along the secretory pathway.Frontiers in endocrinology · 2013Review
- Amperometric measurements at cells support a role for dynamin in the dilation of the fusion pore during exocytosis.Chemphyschem : a European journal of chemical physics and physical chemistry · 2013Article
- B cells use mechanical energy to discriminate antigen affinities.Science (New York, N.Y.) · 2013Article
- Evanescent excitation and emission in fluorescence microscopy.Biophysical journal · 2013Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Assays for real-time investigation of exocytosis typically measure what is released from the granule. From this, inferences are made about the dynamics of membrane remodeling as fusion progresses from start to finish. We have recently undertaken a different approach to investigate the fusion process, by focusing not primarily on the granule, but rather its partner in exocytosis - the plasma membrane. We have been guided by the idea that biochemical interactions between the granule and plasma membranes before and during fusion, cause changes in membrane conformation. To enable study of membrane conformation, a novel imaging technique was developed combining polarized excitation of an oriented membrane probe 1,1'-dioctadecyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (diI) with total internal reflection fluorescence microscopy (pTIRFM). Because this technique measures changes in membrane conformation (or deformations) directly, its usefulness persists even after granule cargo reporter (catecholamine, or protein), is no longer present. In this mini-review, we first summarize the workings of pTIRFM. We then discuss the application of the technique to investigate deformations in the membrane preceding fusion, and later, during fusion pore expansion. Finally, we discuss how expansion of the fusion pore may be regulated by the GTPase activity of dynamin.
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Registered trials
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