ArticleInvestigative ophthalmology & visual science2012
Characterization of fusarium keratitis outbreak isolates: contribution of biofilms to antimicrobial resistance and pathogenesis.
Article in Investigative ophthalmology & visual science, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers, 1 of them a synthesis that pooled it.
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Who cites it
40 citing papers in PubMed, 1 synthesis or guideline pooled it, 99 citations in OpenAlex.
- Impact of biofilm formation in fungal corneal ulcers on treatment outcomes: a systematic review and meta-analysis.Journal of medical microbiology · 2025Pooled it
- HONU: A Multicenter, Prospective, Observational Study of the Progression of Intermediate Age-Related Macular Degeneration.Ophthalmology science · 2026Article
- Morphological Changes in Fungal Elements and Antifungal Drug Susceptibility in an Ex Vivo Model of Fungal Keratitis.Investigative ophthalmology & visual science · 2026Article
- Exogenous Fusarium Endophthalmitis: Clinical Features, Treatment Modalities, and Visual Outcomes.Investigative ophthalmology & visual science · 2026Article
- Optimization of corneal preservation media with novel antifungal agents.Archives of microbiology · 2026Article
- Exploring the agronomic traits, antioxidant and antifungal properties of Hermetia illucens frass extract in durum wheat (Triticum durum Desf.).BMC plant biology · 2025Article
- Antifungal susceptibility, clinical findings, and biofilm resistance of Fusarium species causing keratitis: a challenge for disease control.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2025Article
- Cold atmospheric plasma improves antifungal responsiveness of Aspergillus flavus and Fusarium keratoplasticum conidia and mycelia.PloS one · 2025Article
- Article
- Cold atmospheric plasma inactivatesJournal of medical microbiology · 2024Article
- Fusarioid keratitis and other superficial infections: A 10-years prospective study from Northeastern Brazil.PLoS neglected tropical diseases · 2024Article
- Antifungal susceptibility profiles for fungal isolates from corneas and contact lenses in the United Kingdom.Eye (London, England) · 2024Article
- Outbreaks of Fungal Infections in Hospitals: Epidemiology, Detection, and Management.Journal of fungi (Basel, Switzerland) · 2023Review
- The Role of Biofilms in Contact Lens Associated Fungal Keratitis.Antibiotics (Basel, Switzerland) · 2023Article
- Phase-Dependent Differential In Vitro and Ex Vivo Susceptibility ofJournal of fungi (Basel, Switzerland) · 2023Article
- Candida Biofilm Eye Infection: Main Aspects and Advance in Novel Agents as Potential Source of Treatment.Antibiotics (Basel, Switzerland) · 2023Review
- DectiSomes: C-type lectin receptor-targeted liposomes as pan-antifungal drugs.Advanced drug delivery reviews · 2023Review
- Antimicrobial resistance in topical treatments for microbial keratitis: protocol for a systematic review and meta-analysis.BMJ open · 2023Article
- Endophthalmitis outbreak caused by Fusarium oxysporum after cataract surgery.American journal of ophthalmology case reports · 2022Article
- Recent Advances in Photodynamic Therapy against Fungal Keratitis.Pharmaceutics · 2021Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeFusarium is a major cause of microbial keratitis, and its ability to form biofilms was suggested as a contributing factor in recent outbreaks. We investigated the ability of outbreak Fusarium isolates (F. solani species complex [FSSC] and F. oxysporum species complex [FOSC]) to form biofilms in vitro and in vivo, and evaluated their antifungal susceptibilities.
methodsBiofilm formation was assessed using our in vitro contact lens model and in vivo murine model. Biofilm architecture was assessed using confocal laser scanning microscopy (CLSM). Susceptibility against amphotericin B (AmB), voriconazole (VCZ), and natamycin (NAT) was determined using the CLSI-M38-A2 method and XTT metabolic assay.
resultsFSSC strains formed more biofilms than FOSC, in a strain- and clade-dependent manner. CLSM analyses revealed that "high biofilm forming" (HBF) strains had denser and thicker biofilms than "low biofilm forming" (LBF) strains of both species (thickness 51 vs. 41 μm for FSSC and 61 vs. 45 μm for FOSC strains, P < 0.05 for both comparisons). Fusarium biofilms exhibited species-dependent antifungal susceptibilities (e.g., FSSC biofilms AmB minimal inhibitory concentrations [MIC] ≥16 μg/mL, while NAT or VCZ MICs were 2-8 μg/mL). FSSC-infected mice had severe corneal opacification independent of biofilm thickness, while FOSC infection resulted in moderate corneal opacification. Corneal fungal burden of mice infected with HBF strains was higher than those of the LBF strains. In contrast, the reference ATCC isolate was unable to cause infection.
conclusionsThe ability to form biofilms is a key pathogenicity determinant of Fusarium, irrespective of the thickness of these biofilms. Further studies are warranted to explore this association in greater detail.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.