ArticleMolecular biotechnology2013
The effect of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells.
Article in Molecular biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
17 citing papers in PubMed, 39 citations in OpenAlex.
- Microgravity-induced transcriptional reprogramming in embryonic chicken limb bud-derived chondrogenic cultures.Frontiers in cell and developmental biology · 2026Article
- Artificially altered gravity elicits cell homeostasis imbalance in planarian worms, and cerium oxide nanoparticles counteract this effect.Journal of biomedical materials research. Part A · 2021Article
- Synergistic effects of Indian hedgehog and sonic hedgehog on chondrogenesis during cartilage repair.Journal of molecular histology · 2021Article
- Tissue Engineering of Cartilage Using a Random Positioning Machine.International journal of molecular sciences · 2020Article
- The effects of microgravity on differentiation and cell growth in stem cells and cancer stem cells.Stem cells translational medicine · 2020Article
- Sonic hedgehog promotes chondrogenesis of rabbit bone marrow stem cells in a rotary cell culture system.BMC developmental biology · 2019Article
- Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system.Cellular & molecular biology letters · 2019Article
- Mesenchymal stem cell cultivation in electrospun scaffolds: mechanistic modeling for tissue engineering.Journal of biological physics · 2018Review
- Simulated Microgravity Condition Alters the Gene Expression of some ECM and Adhesion Molecules in Adipose Derived Stem Cells.International journal of molecular and cellular medicine · 2018Article
- Article
- Optimizing Attachment of Human Mesenchymal Stem Cells on Poly(ε-caprolactone) Electrospun Yarns.Journal of visualized experiments : JoVE · 2015Article
- Effects of angular frequency during clinorotation on mesenchymal stem cell morphology and migration.NPJ microgravity · 2015Article
- Barium titanate nanoparticles and hypergravity stimulation improve differentiation of mesenchymal stem cells into osteoblasts.International journal of nanomedicine · 2015Article
- The Effect of OSM on MC3T3-E1 Osteoblastic Cells in Simulated Microgravity with Radiation.PloS one · 2015Article
- The impact of simulated and real microgravity on bone cells and mesenchymal stem cells.BioMed research international · 2014Review
- Article
- Mesenchymal stem cells for cartilage regeneration.Journal of tissue engineeringReview
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We investigated the influence of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells (MSCs). During chondrogenic induction, MSCs combined with polyglycolic acid (PGA) were cultured by static culture or microgravity rotating culture and chondrocyte formation was confirmed by toluidine blue staining. Furthermore, the mRNA and protein expressions of a specific cartilage extracellular matrix protein (collagen type II and Aggrecan) were evaluated by real-time RT-PCR and western blot, respectively. Toluidine blue staining indicated the OD values of proteoglycans semi-determination were higher in the microgravity rotating culture group than the static culture group. Following chondrogenic induction, mRNA and proteins of collagen type II and Aggrecan were more significantly expressed in cells of the microgravity rotating culture group compared with the controls. Compared with routine three-dimensional static culture, the microgravity rotating culture system was more effective for the construction of tissue-engineered cartilage in vitro.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.