ArticleBioTechniques2012
Trehalose-enhanced isolation of neuronal sub-types from adult mouse brain.
Article in BioTechniques, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 59 papers.
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Who cites it
59 citing papers in PubMed.
- A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.Pharmacological reports : PR · 2026Article
- R-loop landscapes in the developing human brain are linked to neural differentiation and cell type-specific transcription.Translational psychiatry · 2026Article
- Distinct sympathetic projections to brown fat regulate thermogenesis and glucose tolerance.Nature metabolism · 2026Article
- SST interneuron maturation extends beyond the second postnatal week and is driven by coordinated transcriptional and functional remodeling.Frontiers in molecular neuroscience · 2026Article
- A spatial single-cell atlas of the claustro-insular region uncovers key regulators of neuronal identity and excitability.Nature communications · 2025Article
- High-Complexity Barcoded Rabies Virus for Scalable Circuit Mapping Using Single-Cell and Single-Nucleus Sequencing.bioRxiv : the preprint server for biology · 2024Article
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- Loss of postnatal Arx transcriptional activity in parvalbumin interneurons reveals non-cell autonomous disturbances in CA1 pyramidal cells.Neuroscience · 2024Article
- Cells and Molecules Underpinning Cannabis-Related Variations in Cortical Thickness during Adolescence.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development.Nature communications · 2024Article
- Light affects the prefrontal cortex via intrinsically photosensitive retinal ganglion cells.Science advances · 2024Article
- Expression of erythropoietin receptor protein in the mouse hippocampus in response to normobaric hypoxia.Heliyon · 2024Article
- Identification of AgRP cells in the murine hindbrain that drive feeding.Molecular metabolism · 2024Article
- Single-cell profiling of glial cells from the mouse amygdala under opioid dependent and withdrawal states.iScience · 2023Article
- Acan downregulation in parvalbumin GABAergic cells reduces spontaneous recovery of fear memories.Molecular psychiatry · 2023Article
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- Article
- Clustering of vomeronasal receptor genes is required for transcriptional stability but not for choice.Science advances · 2022Article
- Optimized Protocol for Proportionate CNS Cell Retrieval as a Versatile Platform for Cellular and Molecular Phenomapping in Aging and Neurodegeneration.International journal of molecular sciences · 2022Article
- A region-dependent allele-biased expression of Dopa decarboxylase in mouse brain.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors.
Funding
Abstract
Efficient isolation of specific, intact, living neurons from the adult brain is problematic due to the complex nature of the extracellular matrix consolidating the neuronal network. Here, we present significant improvements to the protocol for isolation of pure populations of neurons from mature postnatal mouse brain using fluorescence activated cell sorting (FACS). The 10-fold increase in cell yield enables cell-specific transcriptome analysis by protocols such as nanoCAGE and RNA seq.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.