ArticleThe journal of physical chemistry. B2012
Enhanced sensitivity by nonuniform sampling enables multidimensional MAS NMR spectroscopy of protein assemblies.
Article in The journal of physical chemistry. B, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 53 papers.
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Who cites it
53 citing papers in PubMed.
- Super-Resolution solid-state NMR Spectroscopy.Journal of biomolecular NMR · 2026Article
- Evaluating metrics of spectral quality in nonuniform sampling.Journal of magnetic resonance open · 2025Article
- Cross polarization stability in multidimensional NMR spectroscopy of biological solids.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2024Article
- 5D solid-state NMR spectroscopy for facilitated resonance assignment.Journal of biomolecular NMR · 2023Article
- Structural and functional consequences of the H180A mutation of the light-driven sodium pump KR2.Biophysical journal · 2023Article
- Article
- Magic-angle-spinning NMR structure of the kinesin-1 motor domain assembled with microtubules reveals the elusive neck linker orientation.Nature communications · 2022Article
- High fidelity sampling schedules for NMR spectra of high dynamic range.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2022Article
- NMR Assignment of Methyl Groups in Immobilized Proteins Using Multiple-BondFrontiers in molecular biosciences · 2022Article
- Strategies for RNA Resonance Assignment byFrontiers in molecular biosciences · 2021Review
- Solid-state NMR spectroscopy.Nature reviews. Methods primers · 2021Article
- Non-Uniform Sampling in NMR Spectroscopy and the Preservation of Spectral Knowledge in the Time and Frequency Domains.The journal of physical chemistry. A · 2020Article
- Enhancing Compression Level for More Efficient Compressed Sensing and Other Lessons from NMR Spectroscopy.Sensors (Basel, Switzerland) · 2020Article
- Sensitivity boosts by the CPMAS CryoProbe for challenging biological assemblies.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2020Article
- Integrative Protein Modeling in RosettaNMR from Sparse Paramagnetic Restraints.Structure (London, England : 1993) · 2019Article
- Reaching the sparse-sampling limit for reconstructing a single peak in a 2D NMR spectrum using iterated maps.Journal of biomolecular NMR · 2019Article
- Accelerating 2D NMR relaxation dispersion experiments using iterated maps.Journal of biomolecular NMR · 2019Article
- Emerging solution NMR methods to illuminate the structural and dynamic properties of proteins.Current opinion in structural biology · 2019Review
- Global response of diacylglycerol kinase towards substrate binding observed by 2D and 3D MAS NMR.Scientific reports · 2019Article
- Peptide bond conformation in peptides and proteins probed by dipolar coupling-chemical shift tensor correlation solid-state NMR.Journal of magnetic resonance (San Diego, Calif. : 1997) · 2018Article
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Abstract
We report dramatic sensitivity enhancements in multidimensional MAS NMR spectra by the use of nonuniform sampling (NUS) and introduce maximum entropy interpolation (MINT) processing that assures the linearity between the time and frequency domains of the NUS acquired data sets. A systematic analysis of sensitivity and resolution in 2D and 3D NUS spectra reveals that with NUS, at least 1.5- to 2-fold sensitivity enhancement can be attained in each indirect dimension without compromising the spectral resolution. These enhancements are similar to or higher than those attained by the newest-generation commercial cryogenic probes. We explore the benefits of this NUS/MaxEnt approach in proteins and protein assemblies using 1-73-(U-(13)C,(15)N)/74-108-(U-(15)N) Escherichia coli thioredoxin reassembly. We demonstrate that in thioredoxin reassembly, NUS permits acquisition of high-quality 3D-NCACX spectra, which are inaccessible with conventional sampling due to prohibitively long experiment times. Of critical importance, issues that hinder NUS-based SNR enhancement in 3D-NMR of liquids are mitigated in the study of solid samples in which theoretical enhancements on the order of 3-4 fold are accessible by compounding the NUS-based SNR enhancement of each indirect dimension. NUS/MINT is anticipated to be widely applicable and advantageous for multidimensional heteronuclear MAS NMR spectroscopy of proteins, protein assemblies, and other biological systems.
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