ArticlePloS one2012
Experimental inoculation of juvenile rhesus macaques with primate enteric caliciviruses.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
35 citing papers in PubMed, 50 citations in OpenAlex.
- A review of chronic enterocolitis of rhesus macaques (Frontiers in veterinary science · 2026Review
- Human norovirus persists longer than Escherichia coli in sandy soil, independent of plant decaying materials.Scientific reports · 2025Article
- Single-Tube Capture, Concentration, and Genomic Extraction of a Human Norovirus Surrogate Using Magnetic Ionic Liquids.Analytical chemistry · 2025Article
- Multicenter Analysis of the Attributable Diarrhea Risk and Odds Ratios of Pathogens in Rhesus Macaques (Macaca mulatta) Using Multiplex PCR Gastrointestinal Panel Testing and Conventional Fecal Culture.Journal of the American Association for Laboratory Animal Science : JAALAS · 2025Article
- BTP2 restricts Tulane virus and human norovirus replication independent of store-operated calcium entry.Journal of virology · 2025Article
- B cell lines fail to support efficient rhesus enteric calicivirus and human norovirus replication.Journal of virology · 2025Article
- Navigating Uncertainties in RT-qPCR and Infectivity Assessment of Norovirus.Food and environmental virology · 2025Article
- In Vitro Differential Virucidal Efficacy of Alcohol-Based Disinfectants Against Human Norovirus and Its Surrogates.Microorganisms · 2025Article
- Protective Effect of Select Bacterial Species Representative of Fresh Produce on Human Norovirus Surrogates Exposed to Disinfecting Pulsed Light.Applied and environmental microbiology · 2023Article
- Common and Not-So-Common Pathologic Findings of the Gastrointestinal Tract of Rhesus and Cynomolgus Macaques.Toxicologic pathology · 2022Review
- Efficacy and Mechanisms of Copper Ion-Catalyzed Inactivation of Human Norovirus.ACS infectious diseases · 2022Article
- Idiopathic Chronic Diarrhea in Rhesus Macaques Is Not Associated with Enteric Viral Infections.Viruses · 2021Article
- Review
- Animals as Reservoir for Human Norovirus.Viruses · 2019Review
- Non-Human Primate Models of Enteric Viral Infections.Viruses · 2018Review
- Tissue Distribution and Visualization of Internalized Human Norovirus in Leafy Greens.Applied and environmental microbiology · 2018Article
- Case-Control Comparison of Enteric Viromes in Captive Rhesus Macaques with Acute or Idiopathic Chronic Diarrhea.Journal of virology · 2017Article
- Critical role of RIG-I and MDA5 in early and late stages of Tulane virus infection.The Journal of general virology · 2017Article
- Human norovirus binding to select bacteria representative of the human gut microbiota.PloS one · 2017Article
- Abiotic Stress and Phyllosphere Bacteria Influence the Survival of Human Norovirus and Its Surrogates on Preharvest Leafy Greens.Applied and environmental microbiology · 2016Article
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundTissue culture-adapted Tulane virus (TV), a GI.1 rhesus enteric calicivirus (ReCV), and a mixture of GII.2 and GII.4 human norovirus (NoV)-containing stool sample were used to intrastomacheally inoculate juvenile rhesus macaques (Macaca mulatta) in order to evaluate infection caused by these viruses. METHODOLOGY &
findingsTwo of the three TV-inoculated macaques developed diarrhea, fever, virus-shedding in stools, inflammation of duodenum and 16-fold increase of TV-neutralizing (VN) serum antibodies but no vomiting or viremia. No VN-antibody responses could be detected against a GI.2 ReCV strain FT285, suggesting that TV and FT285 represent different ReCV serotypes. Both NoV-inoculated macaques remained asymptomatic but with demonstrable virus shedding in one animal. Examination of duodenum biopsies of the TV-inoculated macaques showed lymphocytic infiltration of the lamina propria and villous blunting. TV antigen-positive (TV+) cells were detected in the lamina propria. In most of the TV+ cells TV co-localized perinuclearly with calnexin--an endoplasmic reticulum protein. A few CD20+TV+ double-positive B cells were also identified in duodenum. To corroborate the authenticity of CD20+TV+ B cells, in vitro cultures of peripheral blood mononuclear cells (PBMCs) from healthy macaques were inoculated with TV. Multicolor flow cytometry confirmed the presence of TV antigen-containing B cells of predominantly CD20+HLA-DR+ phenotype. A 2-log increase of viral RNA by 6 days post inoculation (p<0.05) suggested active TV replication in cultured lymphocytes. CONCLUSIONS/SIGNIFICANCE: Taken together, our results show that ReCVs represent an alternative cell culture and animal model to study enteric calicivirus replication, pathogenesis and immunity.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.