ArticleFrontiers in molecular neuroscience2012
Genetically encoded proton sensors reveal activity-dependent pH changes in neurons.
Article in Frontiers in molecular neuroscience, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 41 citations in OpenAlex.
- A pH-sensitive closed-loop nanomachine to control hyperexcitability at the single neuron level.Nature communications · 2024Article
- Probing intracellular potassium dynamics in neurons with the genetically encoded sensor lc-LysM GEPII 1.0 in vitro and in vivo.Scientific reports · 2024Article
- Effects of extracellular metabolic acidosis on the homeostasis of intracellular pH in hippocampal neurons.Frontiers in physiology · 2024Article
- A genetically targeted ion sensor reveals distinct seizure-related chloride and pH dynamics in GABAergic interneuron populations.iScience · 2023Article
- Role of NKCC1 and KCC2 during hypoxia-induced neuronal swelling in the neonatal neocortex.Neurobiology of disease · 2023Article
- Progress in pH-Sensitive sensors: essential tools for organelle pH detection, spotlighting mitochondrion and diverse applications.Frontiers in pharmacology · 2023Review
- Detection of endogenous NPY release determined by novel GRAB sensor in cultured cortical neurons.Frontiers in cellular neuroscience · 2023Article
- Bidirectional protein-protein interactions control liquid-liquid phase separation of PSD-95 and its interaction partners.iScience · 2022Article
- Regulation of RNA editing by intracellular acidification.Nucleic acids research · 2021Article
- Optical consequences of a genetically-encoded voltage indicator with a pH sensitive fluorescent protein.Neuroscience research · 2019Review
- Engineering Photoactivatability in Genetically Encoded Voltage and pH Indicators.Frontiers in cellular neuroscience · 2019Article
- NTnC-like genetically encoded calcium indicator with a positive and enhanced response and fast kinetics.Scientific reports · 2018Article
- KCC2-dependent Steady-state Intracellular Chloride Concentration and pH in Cortical Layer 2/3 Neurons of Anesthetized and Awake Mice.Frontiers in cellular neuroscience · 2018Article
- Review
- Simultaneous two-photon imaging of intracellular chloride concentration and pH in mouse pyramidal neurons in vivo.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Optical Quantification of Intracellular pH in Drosophila melanogaster Malpighian Tubule Epithelia with a Fluorescent Genetically-encoded pH Indicator.Journal of visualized experiments : JoVE · 2017Article
- Tight Coupling of Astrocyte pH Dynamics to Epileptiform Activity Revealed by Genetically Encoded pH Sensors.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2016Article
- Fluorescent ratiometric pH indicator SypHer2: Applications in neuroscience and regenerative biology.Biochimica et biophysica acta · 2015Article
- Ion dynamics during seizures.Frontiers in cellular neuroscience · 2015Review
- Twenty years of fluorescence imaging of intracellular chloride.Frontiers in cellular neuroscience · 2014Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
The regulation of hydrogen ion concentration (pH) is fundamental to cell viability, metabolism, and enzymatic function. Within the nervous system, the control of pH is also involved in diverse and dynamic processes including development, synaptic transmission, and the control of network excitability. As pH affects neuronal activity, and can also itself be altered by neuronal activity, the existence of tools to accurately measure hydrogen ion fluctuations is important for understanding the role pH plays under physiological and pathological conditions. Outside of their use as a marker of synaptic release, genetically encoded pH sensors have not been utilized to study hydrogen ion fluxes associated with network activity. By combining whole-cell patch clamp with simultaneous two-photon or confocal imaging, we quantified the amplitude and time course of neuronal, intracellular, acidic transients evoked by epileptiform activity in two separate in vitro models of temporal lobe epilepsy. In doing so, we demonstrate the suitability of three genetically encoded pH sensors: deGFP4, E(2)GFP, and Cl-sensor for investigating activity-dependent pH changes at the level of single neurons.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.