ArticleProceedings of the National Academy of Sciences of the United States of America2012
Feedback regulation of plastidic acetyl-CoA carboxylase by 18:1-acyl carrier protein in Brassica napus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 69 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
69 citing papers in PubMed, 152 citations in OpenAlex.
- GWAS-based identification of GmKASII and its interaction with GmFATA1B reveals their role in soybean oil composition and accumulation.The Plant journal : for cell and molecular biology · 2026Article
- Lipid MRI in plant science: principles and potential areas of application.Journal of experimental botany · 2026Review
- Regulatory mechanism of carbohydrate metabolism pathways on oil biosynthesis of oil plantFrontiers in plant science · 2025Article
- REGULATOR OF FATTY ACID SYNTHESIS proteins regulate de novo fatty acid synthesis by modulating hetACCase distribution.The Plant cell · 2024Article
- A bacterial transcription activator dedicated to the expression of the enzyme catalyzing the first committed step in fatty acid biosynthesis.Nucleic acids research · 2024Article
- Targeted engineering of camelina and pennycress seeds for ultrahigh accumulation of acetyl-TAG.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Transcriptome analysis of lipid biosynthesis during kernel development in two walnut (Juglans regia L.) varieties of 'Xilin 3' and 'Xiangling'.BMC plant biology · 2024Article
- Comparative Transcriptomic and Lipidomic Analysis of Fatty Acid Accumulation in ThreeJournal of agricultural and food chemistry · 2024Article
- A telomere-to-telomere gap-free reference genome assembly of avocado provides useful resources for identifying genes related to fatty acid biosynthesis and disease resistance.Horticulture research · 2024Article
- Metabolic and transcriptomic study of pennycress natural variation identifies targets for oil improvement.Plant biotechnology journal · 2023Article
- Alternative carbon sources for the production of plant cellular agriculture: a case study on acetate.Frontiers in plant science · 2023Article
- Biosynthesis of cannabinoid precursor olivetolic acid in genetically engineered Yarrowia lipolytica.Communications biology · 2022Article
- Comparative Proteomics Study on the Postharvest Senescence ofJournal of fungi (Basel, Switzerland) · 2022Article
- Article
- Biotin attachment domain-containing proteins mediate hydroxy fatty acid-dependent inhibition of acetyl CoA carboxylase.Plant physiology · 2021Article
- Chloroplast lipid biosynthesis is fine-tuned to thylakoid membrane remodeling during light acclimation.Plant physiology · 2021Article
- CRISPR/Cas9-InducedFrontiers in plant science · 2021Article
- Integrative Modeling of Gene Expression and Metabolic Networks ofFrontiers in plant science · 2021Article
- Docking of acetyl-CoA carboxylase to the plastid envelope membrane attenuates fatty acid production in plants.Nature communications · 2020Article
- De novo transcriptome analysis of Chlorella sorokiniana: effect of glucose assimilation, and moderate light intensity.Scientific reports · 2020Article
9 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Plant seed oil represents a major renewable source of reduced carbon, but little is known about the biochemical regulation of its synthesis. The goal of this research was to identify potential feedback regulation of fatty acid biosynthesis in Brassica napus embryo-derived cell cultures and to characterize both the feedback signals and enzymatic targets of the inhibition. Fatty acids delivered via Tween esters rapidly reduced the rate of fatty acid synthesis in a dose-dependent and reversible manner, demonstrating the existence of feedback inhibition in an oil-accumulating tissue. Tween feeding did not affect fatty acid elongation in the cytosol or the incorporation of radiolabeled malonate into nascent fatty acids, which together pinpoint plastidic acetyl-CoA carboxylase (ACCase) as the enzymatic target of feedback inhibition. To identify the signal responsible for feedback, a variety of Tween esters were tested for their effects on the rate of fatty acid synthesis. Maximum inhibition was achieved upon feeding oleic acid (18:1) Tween esters that resulted in the intracellular accumulation of 18:1 free fatty acid, 18:1-CoA, and 18:1-acyl-carrier protein (ACP). Direct, saturable inhibition of ACCase enzyme activity was observed in culture extracts and in extracts of developing canola seeds supplemented with 18:1-ACP at physiological concentrations. A mechanism for feedback inhibition is proposed in which reduced demand for de novo fatty acids results in the accumulation of 18:1-ACP, which directly inhibits plastidic ACCase, leading to reduced fatty acid synthesis. Defining this mechanism presents an opportunity for mitigating feedback inhibition of fatty acid synthesis in crop plants to increase oil yield.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.