ArticleProceedings of the National Academy of Sciences of the United States of America2012
Room temperature femtosecond X-ray diffraction of photosystem II microcrystals.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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62 citing papers in PubMed, 165 citations in OpenAlex.
- Sample delivery methods for protein X-ray crystallography with a special focus on sample consumption.Nature communications · 2025Review
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Exploiting fourth-generation synchrotron radiation for enzyme and photoreceptor characterization.IUCrJ · 2025Article
- Lighting the way: Compelling open questions in photosynthesis research.The Plant cell · 2024Article
- Structure Function Studies of Photosystem II Using X-Ray Free Electron Lasers.Annual review of biophysics · 2024Review
- Going around the Kok cycle of the water oxidation reaction with femtosecond X-ray crystallography.IUCrJ · 2023Article
- Does Serial Femtosecond Crystallography Depict State-Specific Catalytic Intermediates of the Oxygen-Evolving Complex?Journal of the American Chemical Society · 2023Article
- Effects of x-ray free-electron laser pulse intensity on the Mn KStructural dynamics (Melville, N.Y.) · 2021Article
- Water oxidation in photosystem II.Photosynthesis research · 2019Review
- Serial Femtosecond X-Ray Diffraction of HIV-1 Gag MA-IP6 Microcrystals at Ambient Temperature.International journal of molecular sciences · 2019Article
- Inception of electronic damage of matter by photon-driven post-ionization mechanisms.Structural dynamics (Melville, N.Y.) · 2019Article
- Structure of the 30S ribosomal decoding complex at ambient temperature.RNA (New York, N.Y.) · 2018Article
- X-ray Emission Spectroscopy as an in Situ Diagnostic Tool for X-ray Crystallography of Metalloproteins Using an X-ray Free-Electron Laser.Biochemistry · 2018Article
- Serial Femtosecond Crystallography of G Protein-Coupled Receptors.Annual review of biophysics · 2018Review
- Serial femtosecond crystallography at the SACLA: breakthrough to dynamic structural biology.Biophysical reviews · 2018Review
- On the relationship between cumulative correlation coefficients and the quality of crystallographic data sets.Protein science : a publication of the Protein Society · 2017Article
- Capturing an initial intermediate during the P450nor enzymatic reaction using time-resolved XFEL crystallography and caged-substrate.Nature communications · 2017Article
- Crystallographic Data Support the Carousel Mechanism of Water Supply to the Oxygen-Evolving Complex of Photosystem II.ACS energy letters · 2017Article
- A Bright Future for Serial Femtosecond Crystallography with XFELs.Trends in biochemical sciences · 2017Review
- Insights into Photosystem II from Isomorphous Difference Fourier Maps of Femtosecond X-ray Diffraction Data and Quantum Mechanics/Molecular Mechanics Structural Models.ACS energy letters · 2017Review
2 more citing papers are in PubMed but not listed here.
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36 authors at 9 institutions in 4 countries.
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Abstract
Most of the dioxygen on earth is generated by the oxidation of water by photosystem II (PS II) using light from the sun. This light-driven, four-photon reaction is catalyzed by the Mn(4)CaO(5) cluster located at the lumenal side of PS II. Various X-ray studies have been carried out at cryogenic temperatures to understand the intermediate steps involved in the water oxidation mechanism. However, the necessity for collecting data at room temperature, especially for studying the transient steps during the O-O bond formation, requires the development of new methodologies. In this paper we report room temperature X-ray diffraction data of PS II microcrystals obtained using ultrashort (< 50 fs) 9 keV X-ray pulses from a hard X-ray free electron laser, namely the Linac Coherent Light Source. The results presented here demonstrate that the "probe before destroy" approach using an X-ray free electron laser works even for the highly-sensitive Mn(4)CaO(5) cluster in PS II at room temperature. We show that these data are comparable to those obtained in synchrotron radiation studies as seen by the similarities in the overall structure of the helices, the protein subunits and the location of the various cofactors. This work is, therefore, an important step toward future studies for resolving the structure of the Mn(4)CaO(5) cluster without any damage at room temperature, and of the reaction intermediates of PS II during O-O bond formation.
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