ArticleProceedings of the National Academy of Sciences of the United States of America2012
Roles of cell confluency and fluid shear in 3-dimensional intracellular forces in endothelial cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed, 133 citations in OpenAlex.
- Modulating cell surface chemistry through mild reduction reinforces extracellular-to-intracellular transmission forces and mechano-signaling.Materials today. Bio · 2026Article
- Cell Surface Thiol Engineering Mechanoregulates Myogenic Differentiation via the FAK-PI3K-AKT Axis.Advanced healthcare materials · 2026Article
- Regulation of chromatin modifications through coordination of nucleus size and epithelial cell morphology heterogeneity.Communications biology · 2025Article
- Transient low shear-stress preconditioning influences long-term endothelial traction and alignment under high shear flow.American journal of physiology. Heart and circulatory physiology · 2024Article
- Predicting Lipid-Rich Plaque Progression in Coronary Arteries Using Multimodal Imaging and Wall Shear Stress Signatures.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Low or oscillatory shear stress and endothelial permeability in atherosclerosis.Frontiers in physiology · 2024Review
- Computational analysis of cancer cell adhesion in curved vessels affected by wall shear stress for prediction of metastatic spreading.Frontiers in bioengineering and biotechnology · 2024Article
- Early committed polarization of intracellular tension in response to cell shape determines the osteogenic differentiation of mesenchymal stromal cells.Acta biomaterialia · 2023Article
- Competition for endothelial cell polarity drives vascular morphogenesis in the mouse retina.Developmental cell · 2022Article
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- Mechanical Forces Govern Interactions of Host Cells with Intracellular Bacterial Pathogens.Microbiology and molecular biology reviews : MMBR · 2022Review
- Biomechanical interactions of Schistosoma mansoni eggs with vascular endothelial cells facilitate egg extravasation.PLoS pathogens · 2022Article
- Optimization of Mechanical Tissue Dissociation Using an Integrated Microfluidic Device for Improved Generation of Single Cells Following Digestion.Frontiers in bioengineering and biotechnology · 2022Article
- Human endothelial cells display a rapid tensional stress increase in response to tumor necrosis factor-α.PloS one · 2022Article
- Early Atherosclerotic Changes in Coronary Arteries are Associated with Endothelium Shear Stress Contraction/Expansion Variability.Annals of biomedical engineering · 2021Article
- Editorial: Understanding molecular interactions that underpin vascular mechanobiology.APL bioengineering · 2021Article
- Review
- Integration of substrate- and flow-derived stresses in endothelial cell mechanobiology.Communications biology · 2021Review
- Volume measurement and biophysical characterization of mounds in epithelial monolayers after intracellular bacterial infection.STAR protocols · 2021Article
- Ameliorating Fibrotic Phenotypes of Keloid Dermal Fibroblasts through an Epidermal Growth Factor-Mediated Extracellular Matrix Remodeling.International journal of molecular sciences · 2021Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
11 authors at 4 institutions in 3 countries.
Funding
Abstract
We use a novel 3D inter-/intracellular force microscopy technique based on 3D traction force microscopy to measure the cell-cell junctional and intracellular tensions in subconfluent and confluent vascular endothelial cell (EC) monolayers under static and shear flow conditions. We found that z-direction cell-cell junctional tensions are higher in confluent EC monolayers than those in subconfluent ECs, which cannot be revealed in the previous 2D methods. Under static conditions, subconfluent cells are under spatially non-uniform tensions, whereas cells in confluent monolayers are under uniform tensions. The shear modulations of EC cytoskeletal remodeling, extracellular matrix (ECM) adhesions, and cell-cell junctions lead to significant changes in intracellular tensions. When a confluent monolayer is subjected to flow shear stresses with a high forward component comparable to that seen in the straight part of the arterial system, the intracellular and junction tensions preferentially increase along the flow direction over time, which may be related to the relocation of adherens junction proteins. The increases in intracellular tensions are shown to be a result of chemo-mechanical responses of the ECs under flow shear rather than a direct result of mechanical loading. In contrast, the intracellular tensions do not show a preferential orientation under oscillatory flow with a very low mean shear. These differences in the directionality and magnitude of intracellular tensions may modulate translation and transcription of ECs under different flow patterns, thus affecting their susceptibility for atherogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.