ArticleProceedings of the National Academy of Sciences of the United States of America2012
Wet-surface-enhanced ellipsometric contrast microscopy identifies slime as a major adhesion factor during bacterial surface motility.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed, 80 citations in OpenAlex.
- High-Throughput Ellipsometric Contrast Microscopy of Lateral 2D Heterostructures for Optoelectronics.Small methods · 2026Article
- Secretins of type-two secretion systems are necessary for exopolymeric slime secretion in cyanobacteria and myxobacteria.Nature communications · 2025Article
- Milestones in the development ofJournal of bacteriology · 2025Review
- Fast Twist Angle Mapping of Bilayer Graphene Using Spectroscopic Ellipsometric Contrast Microscopy.Nano letters · 2023Article
- Unmasking of the von Willebrand A-domain surface adhesin CglB at bacterial focal adhesions mediates myxobacterial gliding motility.Science advances · 2023Article
- Bacterial Outer Membrane Polysaccharide Export (OPX) Proteins Occupy Three Structural Classes with Selective β-Barrel Porin Requirements for Polymer Secretion.Microbiology spectrum · 2022Article
- Evaluation of Azido 3-Deoxy-d-ACS omega · 2022Article
- Bacterial Glycocalyx Integrity Impacts Tolerance ofBiomolecules · 2022Article
- The environment topography alters the way to multicellularity inScience advances · 2021Article
- Modulation of bacterial multicellularity via spatio-specific polysaccharide secretion.PLoS biology · 2020Article
- Enhanced avidin binding to lipid bilayers using PDP-PE lipids with PEG-biotin linkers.Nanoscale advances · 2020Article
- Mechanisms for bacterial gliding motility on soft substrates.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Physiology, Metabolism, and Fossilization of Hot-Spring Filamentous Microbial Mats.Astrobiology · 2019Article
- Antimicrobial Peptides: Powerful Biorecognition Elements to Detect Bacteria in Biosensing Technologies.Molecules (Basel, Switzerland) · 2018Review
- Bacterial Surface Spreading Is More Efficient on Nematically Aligned Polysaccharide Substrates.Journal of bacteriology · 2018Article
- Assessing Travel Conditions: Environmental and Host Influences On Bacterial Surface Motility.Journal of bacteriology · 2018Article
- Live nanoscopic to mesoscopic topography reconstruction with an optical microscope for chemical and biological samples.PloS one · 2018Article
- Polymertropism of rod-shaped bacteria: movement along aligned polysaccharide fibers.Scientific reports · 2017Article
- Force generation by groups of migrating bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In biology, the extracellular matrix (ECM) promotes both cell adhesion and specific recognition, which is essential for central developmental processes in both eukaryotes and prokaryotes. However, live studies of the dynamic interactions between cells and the ECM, for example during motility, have been greatly impaired by imaging limitations: mostly the ability to observe the ECM at high resolution in absence of specific staining by live microscopy. To solve this problem, we developed a unique technique, wet-surface enhanced ellipsometry contrast (Wet-SEEC), which magnifies the contrast of transparent organic materials deposited on a substrate (called Wet-surf) with exquisite sensitivity. We show that Wet-SEEC allows both the observation of unprocessed nanofilms as low as 0.2 nm thick and their accurate 3D topographic reconstructions, directly by standard light microscopy. We next used Wet-SEEC to image slime secretion, a poorly defined property of many prokaryotic and eukaryotic organisms that move across solid surfaces in absence of obvious extracellular appendages (gliding). Using combined Wet-SEEC and fluorescent-staining experiments, we observed slime deposition by gliding Myxococcus xanthus cells at unprecedented resolution. Altogether, the results revealed that in this bacterium, slime associates preferentially with the outermost components of the motility machinery and promotes its adhesion to the substrate on the ventral side of the cell. Strikingly, analogous roles have been proposed for the extracellular proteoglycans of gliding diatoms and apicomplexa, suggesting that slime deposition is a general means for gliding organisms to adhere and move over surfaces.
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