Evidence map›Paper›PMID 22665611›Full record

ArticleThe American journal of tropical medicine and hygiene2012

Facilitated molecular typing of Shigella isolates using ERIC-PCR.

Margaret Kosek, Pablo Peñataro Yori, Robert H Gilman, Henry Vela, Maribel Paredes Olortegui, Cesar Banda Chavez, Maritza Calderon, Juan Perez Bao, Eric Hall, Ryan Maves and 2 more

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in The American journal of tropical medicine and hygiene, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Occurrence of blaBrazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  2. Article
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  6. Article
  7. Burden, Antibiotic Resistance, and Clonality ofTropical medicine and infectious disease · 2021
    Article
  8. Article
  9. A high number of multidrug-resistant and predominant genetically related cluster of Shigella flexneri strains isolated over 34 years in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020
    Article
  10. Molecular determination of genetic diversity amongInfection ecology & epidemiology · 2020
    Article
  11. First investigation of the presence ofInfection and drug resistance · 2019
    Article
  12. The journal of poultry science · 2018
    Article
  13. Article
  14. Article
  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Margaret KosekJohns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA. mkosek@jhsph.edu
Pablo Peñataro Yori
Robert H Gilman
Henry Vela
Maribel Paredes Olortegui
Cesar Banda Chavez
Maritza Calderon
Juan Perez Bao
Eric Hall
Ryan Maves
Rosa Burga
Graciela Meza Sanchez

Funding

Epidemiology of Shigellosis in the Peruvian AmazonK01TW005717 · FIC · JOHNS HOPKINS UNIVERSITY · PI KOSEK, MARGARET N · 2001 to 2007
$615k
FIC NIH HHS K01 TW005717FIC NIH HHS K01TW5717
6 · The paper itself

Abstract

To evaluate the performance of enterobacterial repetitive intergenic sequence-based polymerase chain reaction (ERIC-PCR) typing versus the current standard for the typing of Shigella pulsed gel electrophoresis (PFGE), we typed 116 Shigella isolates from a village in an endemic setting over a 20-month period using both methods. PFGE identified 37 pulse types and had a discrimination index of 0.925 (95% confidence interval = 0.830-1.00), whereas ERIC-PCR identified 42 types and had a discrimination index of 0.961 (95% confidence interval = 0.886-1.00). PFGE and ERIC-PCR showed a 90.4% correlation in the designation of isolates as clonal or non-clonal in pairwise comparisons. Both systems were highly reproducible and provided highly similar and supplementary data compared with serotyping regarding the transmission dynamics of shigellosis in this community. ERIC-PCR is considerably more rapid and inexpensive than PFGE and may have a complementary role to PFGE for initial investigations of hypothesized outbreaks in resource-limited settings.

Indexed as

Repetitive Sequences, Nucleic AcidBacterial Typing TechniquesDiarrheaDNA, BacterialDNA, IntergenicDysentery, BacillaryElectrophoresis, Gel, Pulsed-FieldHumansPolymerase Chain ReactionPrevalenceSerotypingShigellaDNA, BacterialDNA, Intergenic

Identifiers

PMID22665611
PMCPMC3366515

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.