Evidence map›Paper›PMID 22665590›Full record

ArticleCBE life sciences education2012

A simple microscopy assay to teach the processes of phagocytosis and exocytosis.

Ross Gray, Andrew Gray, Jessica L Fite, Renée Jordan, Sarah Stark, Kari Naylor

Open access · hybridAbstract read
In one paragraph

Article in CBE life sciences education, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.2field-weighted citation impact, top 43% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 10 citations in OpenAlex.

  1. Review
  2. Classroom-Based Research Experiences to Support Underserved STEM Student Success: From Introductory Inquiry to Optogenetics in the Embryonic Chicken.Journal of undergraduate neuroscience education : JUNE : a publication of FUN, Faculty for Undergraduate Neuroscience · 2018
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 1 country.

Ross GrayDepartment of Biology, University of Central Arkansas, Conway, AR 72034, USA.
Andrew Gray
Jessica L Fite
Renée Jordan
Sarah Stark
Kari Naylor
University of Central Arkansas · USConway School of Landscape Design · US

Funding

VIRULENCE MECHANISMS IN ESCHERICHIA COLIP20RR016460 · NCRR · UNIV OF ARKANSAS FOR MED SCIS · PI HENSLEY, LORI · 2001 to 2011
$33.1M
NCRR NIH HHS P20 RR016460NCRR NIH HHS P20RR16460
6 · The paper itself

Abstract

Phagocytosis and exocytosis are two cellular processes involving membrane dynamics. While it is easy to understand the purpose of these processes, it can be extremely difficult for students to comprehend the actual mechanisms. As membrane dynamics play a significant role in many cellular processes ranging from cell signaling to cell division to organelle renewal and maintenance, we felt that we needed to do a better job of teaching these types of processes. Thus, we developed a classroom-based protocol to simultaneously study phagocytosis and exocytosis in Tetrahymena pyriformis. In this paper, we present our results demonstrating that our undergraduate classroom experiment delivers results comparable with those acquired in a professional research laboratory. In addition, students performing the experiment do learn the mechanisms of phagocytosis and exocytosis. Finally, we demonstrate a mathematical exercise to help the students apply their data to the cell. Ultimately, this assay sets the stage for future inquiry-based experiments, in which the students develop their own experimental questions and delve deeper into the mechanisms of phagocytosis and exocytosis.

Indexed as

ExocytosisPhagocytosisTeachingActin CytoskeletonBiological AssayBridged Bicyclo Compounds, HeterocyclicCell BiologyCell MembraneColchicineCytoplasmic VesiclesEducational MeasurementMicroscopyMicrotubulesTetrahymenaThiazolidinesBridged Bicyclo Compounds, HeterocyclicColchicinelatrunculin AThiazolidines

Identifiers

PMID22665590
PMCPMC3366903
OpenAlexW2104386211

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-SA
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.