ArticleJournal of visualized experiments : JoVE2012
Isolation and culture of rat embryonic neural cells: a quick protocol.
Article in Journal of visualized experiments : JoVE, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed.
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- Tunneling nanotubes induced by pseudorabies virus facilitate viral transmission in neuronal cells to evade the immune system.Cell communication and signaling : CCS · 2026Article
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- Establishment of a Depression Model Using Dexamethasone-treated Three-dimensional Cultured Rat Cortical Cells.Clinical psychopharmacology and neuroscience : the official scientific journal of the Korean College of Neuropsychopharmacology · 2025Article
- Development of NIR photocleavable nanoparticles with BDNF for vestibular neuron regeneration.Journal of nanobiotechnology · 2025Article
- Long-term functional rescue of trauma-induced vision loss by a novel, small molecule TrkB modulator.PloS one · 2025Article
- Calcineurin-mediated regulation of growth-associated protein 43 is essential for neurite and synapse formation and protects against α-synuclein-induced degeneration.Frontiers in aging neuroscience · 2025Article
- IL-33 relieves nerve injury by mediating microglial polarization in neuromyelitis optica spectrum disorders via the IL-33/ST2 pathway.IBRO neuroscience reports · 2024Article
- Ubiquitin-like 4A alleviates the progression of intracerebral hemorrhage by regulating oxidative stress and mitochondrial damage.Experimental animals · 2024Article
- Targeting ferroptosis with the lipoxygenase inhibitor PTC-041 as a therapeutic strategy for the treatment of Parkinson's disease.PloS one · 2024Article
- Synapses without tension fail to fire in an in vitro network of hippocampal neurons.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Extracellular matrix composition affects outgrowth of dendrites and dendritic spines on cortical neurons.Frontiers in cellular neuroscience · 2023Article
- Neuronal Puncta/Aggregate Formation by WT and Mutant UBQLN2.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Wireless Force-Inducing Neuronal Stimulation Mediated by High Magnetic Moment Microdiscs.Advanced healthcare materials · 2022Article
- Canonical Bone Morphogenetic Protein Signaling Regulates Expression of Aquaporin-4 and Its Anchoring Complex in Mouse Astrocytes.Frontiers in cellular neuroscience · 2022Article
- C9orf72 regulates the unfolded protein response and stress granule formation by interacting with eIF2α.Theranostics · 2022Article
- Label-free monitoring of 3D cortical neuronal growthBiomedical optics express · 2021Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
We are describing a quick method to dissociate and culture hippocampal or cortical neurons from E15-17 rat embryos. The procedure can be applied successfully to the isolation of mouse and human primary neurons and neural progenitors. Dissociated neurons are maintained in serum-free medium up to several weeks. These cultures can be used for nucleofection, immunocytochemistry, nucleic acids preparation, as well as electrophysiology. Older neuronal cultures can also be transfected with a good efficiency rate by lentiviral transduction and, less efficiently, with calcium phosphate or lipid-based methods such as lipofectamine.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.