ArticlePloS one2012
Identification, expression and target gene analyses of Micrornas in Spodoptera litura.
Article in PloS one, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
8 citing papers in PubMed, 29 citations in OpenAlex.
- Differential gene expression and microRNA profile in corpora allata-corpora cardiaca of Aedes aegypti mosquitoes with weak juvenile hormone signalling.BMC genomics · 2024Article
- The Role of MicroRNAs in Genome Response to Plant-Lepidoptera Interaction.Plants (Basel, Switzerland) · 2019Review
- A systemic study of indoxacarb resistance in Spodoptera litura revealed complex expression profiles and regulatory mechanism.Scientific reports · 2019Article
- Identification and characteristics of microRNAs from army worm, Spodoptera frugiperda cell line Sf21.PloS one · 2015Article
- Emerging strategies for RNA interference (RNAi) applications in insects.Bioengineered · 2015Review
- Discovery of mosquito saliva microRNAs during CHIKV infection.PLoS neglected tropical diseases · 2015Article
- Identification of microRNAs in Helicoverpa armigera and Spodoptera litura based on deep sequencing and homology analysis.International journal of biological sciences · 2013Article
- Development associated profiling of chitinase and microRNA of Helicoverpa armigera identified chitinase repressive microRNA.Scientific reports · 2013Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MicroRNAs (miRNAs) are small RNAs widely present in animals and plants and involved in post-transcriptional regulation of gene transcripts. In this study we identified and validated 58 miRNAs from an EST dataset of Spodoptera litura based on the computational and experimental analysis of sequence conservation and secondary structure of miRNA by comparing the miRNA sequences in the miRbase. RT-PCR was conducted to examine the expression of these miRNAs and stem-loop RT-PCR assay was performed to examine expression of 11 mature miRNAs (out of the 58 putative miRNA) that showed significant changes in different tissues and stages of the insect development. One hundred twenty eight possible target genes against the 11 miRNAs were predicted by using computational methods. Binding of one miRNA (sli-miR-928b) with the three possible target mRNAs was confirmed by Southern blotting, implying its possible function in regulation of the target genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.