ArticleBiomicrofluidics2012
Gravity-oriented microfluidic device for uniform and massive cell spheroid formation.
Article in Biomicrofluidics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 54 citations in OpenAlex.
- Organoid-based tissue engineering for advanced tissue repair and reconstruction.Materials today. Bio · 2025Review
- Human-Derived Cortical Neurospheroids Coupled to Passive, High-Density and 3D MEAs: A Valid Platform for Functional Tests.Bioengineering (Basel, Switzerland) · 2023Article
- Review
- From cell spheroids to vascularized cancer organoids: Microfluidic tumor-on-a-chip models for preclinical drug evaluations.Biomicrofluidics · 2021Review
- Simultaneous Droplet Generation with In-Series Droplet T-Junctions Induced by Gravity-Induced Flow.Micromachines · 2021Article
- Computational Modelling and Big Data Analysis of Flow and Drug Transport in Microfluidic Systems: A Spheroid-on-a-Chip Study.Frontiers in bioengineering and biotechnology · 2021Article
- Using Spheroids as Building Blocks Towards 3D Bioprinting of Tumor Microenvironment.International journal of bioprinting · 2021Review
- Microfluidic technologies to engineer mesenchymal stem cell aggregates-applications and benefits.Biophysical reviews · 2020Review
- 3D printing approaches for cardiac tissue engineering and role of immune modulation in tissue regeneration.International journal of nanomedicine · 2019Review
- Developments with 3D bioprinting for novel drug discovery.Expert opinion on drug discovery · 2018Review
- Analysis of Cancer Cell Invasion and Anti-metastatic Drug Screening Using Hydrogel Micro-chamber Array (HMCA)-based Plates.Journal of visualized experiments : JoVE · 2018Article
- High-Throughput Cancer Cell Sphere Formation for Characterizing the Efficacy of Photo Dynamic Therapy in 3D Cell Cultures.Scientific reports · 2015Article
- Migration and vascular lumen formation of endothelial cells in cancer cell spheroids of various sizes.Biomicrofluidics · 2014Article
- Micro total analysis systems: fundamental advances and applications in the laboratory, clinic, and field.Analytical chemistry · 2013Review
- Microfluidic device for trapping and monitoring three dimensional multicell spheroids using electrical impedance spectroscopy.Biomicrofluidics · 2013Article
- Empirical chemosensitivity testing in a spheroid model of ovarian cancer using a microfluidics-based multiplex platform.Biomicrofluidics · 2013Article
- A microfluidic device for uniform-sized cell spheroids formation, culture, harvesting and flow cytometry analysis.Biomicrofluidics · 2013Article
- On-chip three-dimensional tumor spheroid formation and pump-less perfusion culture using gravity-driven cell aggregation and balanced droplet dispensing.Biomicrofluidics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We propose a simple method for forming massive and uniform three-dimensional (3-D) cell spheroids in a multi-level structured microfluidic device by gravitational force. The concept of orienting the device vertically has allowed spheroid formation, long-term perfusion, and retrieval of the cultured spheroids by user-friendly standard pipetting. We have successfully formed, perfused, and retrieved uniform, size-controllable, well-conditioned spheroids of human embryonic kidney 293 cells (HEK 293) in the gravity-oriented microfluidic device. We expect the proposed method will be a useful tool to study in-vitro 3-D cell models for the proliferation, differentiation, and metabolism of embryoid bodies or tumours.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.