ArticleBiomicrofluidics2012
Integrated microfluidic chip for rapid DNA digestion and time-resolved capillary electrophoresis analysis.
Article in Biomicrofluidics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
5 citing papers in PubMed, 36 citations in OpenAlex.
- Ensuring food safety: Microfluidic-based approaches for the detection of food contaminants.Analytical science advances · 2024Review
- A multiplexed immunoaggregation biomarker assay using a two-stage micro resistive pulse sensor.Biomicrofluidics · 2016Article
- A hydrodynamic focusing microchannel based on micro-weir shear lift force.Biomicrofluidics · 2012Article
- High-performance microfluidic rectifier based on sudden expansion channel with embedded block structure.Biomicrofluidics · 2012Article
- Preface to special topic: selected papers from the second conference on advances in microfluidics and nanofluidics and Asia-pacific international symposium on lab on chip.Biomicrofluidics · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An integrated microfluidic chip is proposed for rapid DNA digestion and time-resolved capillary electrophoresis (CE) analysis. The chip comprises two gel-filled chambers for DNA enrichment and purification, respectively, a T-form micromixer for DNA/restriction enzyme mixing, a serpentine channel for DNA digestion reaction, and a CE channel for on-line capillary electrophoresis analysis. The DNA and restriction enzyme are mixed electroomostically using a pinched-switching DC field. The experimental and numerical results show that a mixing performance of 97% is achieved within a distance of 1 mm from the T-junction when a driving voltage of 90 V/cm and a switching frequency of 4 Hz are applied. Successive mixing digestion and capillary electrophoresis operation clearly present the changes on digesting φx-174 DNA in different CE runs. The time-resolved electropherograms show that the proposed device enables a φx-174 DNA sample comprising 11 fragments to be concentrated and analyzed within 24 min. Overall, the results presented in this study show that the proposed microfluidic chip provides a rapid and effective tool for DNA digestion and CE analysis applications.
Identifiers
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.