ArticlePLoS genetics2012
DNA demethylation and USF regulate the meiosis-specific expression of the mouse Miwi.
Article in PLoS genetics, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
19 citing papers in PubMed, 41 citations in OpenAlex.
- Comparative evaluation of germ cell-specific promoters identifies robust drivers of transgene expression during mouse spermatogenesis.Molecular biology reports · 2026Article
- Testis-specific protein HSF5 is essential for proper chromatin organization and transcriptional reprogramming to drive pachynema progression.Zoological research · 2025Article
- DNA methylation modification is associated with gonadal differentiation in Monopterus albus.Cell & bioscience · 2020Article
- Haploinsufficiency of GCP4 induces autophagy and leads to photoreceptor degeneration due to defective spindle assembly in retina.Cell death and differentiation · 2020Article
- Ectopic Methylation of a Single Persistently Unmethylated CpG in the Promoter of the Vitellogenin Gene Abolishes Its Inducibility by Estrogen through Attenuation of Upstream Stimulating Factor Binding.Molecular and cellular biology · 2019Article
- DNA methylation status of TBX20 in patients with tetralogy of Fallot.BMC medical genomics · 2019Article
- Loss-of-function of sox3 causes follicle development retardation and reduces fecundity in zebrafish.Protein & cell · 2019Article
- Dynamic reorganization of open chromatin underlies diverse transcriptomes during spermatogenesis.Nucleic acids research · 2018Article
- Regulatory elements and transcriptional control of chickenJournal of animal science and biotechnology · 2017Article
- DNA methylation-mediated transcription factors regulate Piwil1 expression during chicken spermatogenesis.The Journal of reproduction and development · 2016Article
- Methylation of the Gpat2 promoter regulates transient expression during mouse spermatogenesis.The Biochemical journal · 2015Article
- Association of single nucleotide polymorphisms in the USF1, GTF2A1L and OR2W3 genes with non-obstructive azoospermia in the Chinese population.Journal of assisted reproduction and genetics · 2015Article
- Article
- MYBL2 guides autophagy suppressor VDAC2 in the developing ovary to inhibit autophagy through a complex of VDAC2-BECN1-BCL2L1 in mammals.Autophagy · 2015Article
- Article
- DNA methylation of heparanase promoter influences its expression and associated with the progression of human breast cancer.PloS one · 2014Article
- Piwi Proteins and piRNAs step onto the systems biology stage.Advances in experimental medicine and biology · 2014Article
- Identification and characterization of germ cell genes expressed in the F9 testicular teratoma stem cell line.PloS one · 2014Article
- Cloning and expression characterization of the chicken Piwil1 gene.Molecular biology reports · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Miwi, a member of the Argonaute family, is required for initiating spermiogenesis; however, the mechanisms that regulate the expression of the Miwi gene remain unknown. By mutation analysis and transgenic models, we identified a 303 bp proximal promoter region of the mouse Miwi gene, which controls specific expression from midpachytene spermatocytes to round spermatids during meiosis. We characterized the binding sites of transcription factors NF-Y (Nuclear Factor Y) and USF (Upstream Stimulatory Factor) within the core promoter and found that both factors specifically bind to and activate the Miwi promoter. Methylation profiling of three CpG islands within the proximal promoter reveals a markedly inverse correlation between the methylation status of the CpG islands and germ cell type-specific expression of Miwi. CpG methylation at the USF-binding site within the E2 box in the promoter inhibits the binding of USF. Transgenic Miwi-EGFP and endogenous Miwi reveal a subcellular co-localization pattern in the germ cells of the Miwi-EGFP transgenic mouse. Furthermore, the DNA methylation profile of the Miwi promoter-driven transgene is consistent with that of the endogenous Miwi promoter, indicating that Miwi transgene is epigenetically modified through methylation in vivo to ensure its spatio-temporal expression. Our findings suggest that USF controls Miwi expression from midpachytene spermatocytes to round spermatids through methylation-mediated regulation. This work identifies an epigenetic regulation mechanism for the spatio-temporal expression of mouse Miwi during spermatogenesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.