ArticleTransgenic research2013
Expression of biologically active human interferon alpha 2b in the milk of transgenic mice.
Article in Transgenic research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Competent expression of effective and long-acting human α-Gal A-Fc fusion protein in the milk of transgenic mice.Transgenic research · 2025Article
- Construction and Identification of a Breast Bioreactor for Human-Derived Hypoglycemic Protein Amylin.Life (Basel, Switzerland) · 2024Article
- A potential paradigm in CRISPR/Cas systems delivery: at the crossroad of microalgal gene editing and algal-mediated nanoparticles.Journal of nanobiotechnology · 2023Review
- Evaluation of the α-casein (CSN1S1) locus as a potential target for a site-specific transgene integration.Scientific reports · 2022Article
- Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland.Bioscience reports · 2019Article
- Engineering Chlamydomonas reinhardtii for Expression of Functionally Active Human Interferon-α.Molecular biotechnology · 2019Article
- Avian Bioreactor Systems: A Review.Molecular biotechnology · 2018Review
- Production of functional human CuZn-SOD and EC-SOD in bitransgenic cloned goat milk.Transgenic research · 2018Article
- The development of modified human Hsp70 (HSPA1A) and its production in the milk of transgenic mice.Cell stress & chaperones · 2016Article
- Intron V, not intron I of human thrombopoietin, improves expression in the milk of transgenic mice regulated by goat beta-casein promoter.Scientific reports · 2015Article
- Production of Recombinant Proteins in the Milk of Transgenic Animals: Current State and Prospects.Acta naturaeArticle
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Interferon alpha 2b (IFNα-2b) is an important immune regulator widely used in clinic, for the treatment of chronic hepatitis, hairy cell leukemia, chronic myelogenous leukemia and multiple myeloma, etc. The clinically used IFNα-2b is generally produced by E.Coli, which lacks the post-translational O-glycosylation presents on naturally synthesized protein, and has a short serum half-life. In this study, a transgenic cassette pBCN-IFN-pA-CMV-EGFP was constructed, with a 5.2 kb beta-casein regulation fragment from Jersey cow and a 6×His tagged human Interferon alpha 2b (hIFNα-2b) gene fragment. By using pronuclear microinjection technique, transgenic mice were generated and the expression of IFNα-2b in the milk was assayed. The hIFNα-2b was correctly translated in milk of transgenic mice according to Western blot analysis. The expression level of hIFNα-2b was varied among the transgenic mice, and the highest one was about 29.71 μg/L. The recombinant protein exhibited biological activity in vitro by increasing the luminescence value and the MxA gene expression in established WISH cells, and the specific activity is approximately 2.8 × 10(7 )IU/mg. The expression of recombinant hIFNα-2b in mammary glands of transgenic mice constitutes an important step towards low-cost and full biological activity production of this protein drug in mammary gland bioreactor.
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