ArticleProceedings of the National Academy of Sciences of the United States of America2012
Modeling disease mutations by gene targeting in one-cell mouse embryos.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it, 55 citations in OpenAlex.
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- Establishment of a Cre-rat resource for creating conditional and physiological relevant models of human diseases.Transgenic research · 2021Article
- Development of a Reporter System to Explore MMEJ in the Context of Replacing Large Genomic Fragments.Molecular therapy. Nucleic acids · 2018Article
- MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems.Nature protocols · 2016Article
- Induced Pluripotency and Gene Editing in Disease Modelling: Perspectives and Challenges.International journal of molecular sciences · 2015Review
- Controlled delivery of β-globin-targeting TALENs and CRISPR/Cas9 into mammalian cells for genome editing using microinjection.Scientific reports · 2015Article
- Homology-directed repair in rodent zygotes using Cas9 and TALEN engineered proteins.Scientific reports · 2015Article
- Fitness Assays Reveal Incomplete Functional Redundancy of the HoxA1 and HoxB1 Paralogs of Mice.Genetics · 2015Article
- Development of an intein-mediated split-Cas9 system for gene therapy.Nucleic acids research · 2015Article
- Efficient introgression of allelic variants by embryo-mediated editing of the bovine genome.Scientific reports · 2015Article
- From hacking the human genome to editing organs.Organogenesis · 2015Review
- Creation of targeted genomic deletions using TALEN or CRISPR/Cas nuclease pairs in one-cell mouse embryos.FEBS open bio · 2015Article
- Efficient bi-allelic gene knockout and site-specific knock-in mediated by TALENs in pigs.Scientific reports · 2014Article
- Efficient gene targeting by homology-directed repair in rat zygotes using TALE nucleases.Genome research · 2014Article
- Mouse genome engineering using designer nucleases.Journal of visualized experiments : JoVE · 2014Article
- Genetically engineered humanized mouse models for preclinical antibody studies.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2014Review
- Modeling human epilepsy by TALEN targeting of mouse sodium channel Scn8a.Genesis (New York, N.Y. : 2000) · 2014Article
- TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS (MVA) syndrome.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene targeting by zinc-finger nucleases in one-cell embryos provides an expedite mutagenesis approach in mice, rats, and rabbits. This technology has been recently used to create knockout and knockin mutants through the deletion or insertion of nucleotides. Here we apply zinc-finger nucleases in one-cell mouse embryos to generate disease-related mutants harboring single nucleotide or codon replacements. Using a gene-targeting vector or a synthetic oligodesoxynucleotide as template for homologous recombination, we introduced missense and silent mutations into the Rab38 gene, encoding a small GTPase that regulates intracellular vesicle trafficking. These results demonstrate the feasibility of seamless gene editing in one-cell embryos to create genetic disease models and establish synthetic oligodesoxynucleotides as a simplified mutagenesis tool.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.