ArticleFish physiology and biochemistry2012
Establishment and characterization of a new fish cell line from head kidney of half-smooth tongue sole (Cynoglossus semilaevis).
Article in Fish physiology and biochemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
14 citing papers in PubMed.
- Ninjurin-1 executes plasma membrane rupture and impairs anti-microbial immunity in an early jawed vertebrate.Cellular and molecular life sciences : CMLS · 2025Article
- Unveiling Gene Expression Dynamics during Early Embryogenesis inLife (Basel, Switzerland) · 2024Article
- CircRNA Identification and CircRNA-miRNA-mRNA Network inBiology · 2022Article
- An established kidney cell line from humpback grouper (Cromileptes altivelis) and its susceptibility to bacteria and heavy metals.Fish physiology and biochemistry · 2022Article
- Genomics and transcriptomics reveal new molecular mechanism of vibriosis resistance in fish.Frontiers in immunology · 2022Article
- Establishment, characterization, and transfection potential of a new continuous fish cell line (CAM) derived from the muscle tissue of grass goldfish (Carassius auratus).In vitro cellular & developmental biology. Animal · 2021Article
- Establishment of a new fish cell line from the brain of humpback grouper (Cromileptes altivelis) and its application in toxicology and bacterial susceptibility.Fish physiology and biochemistry · 2021Article
- Establishment and characterization of a kidney cell line from kelp grouper Epinephelus moara.Fish physiology and biochemistry · 2018Article
- Two skin cell lines from wild-type and albino Japanese flounder (Paralichthys olivaceus): establishment, characterization, virus susceptibility, efficient transfection, and application to albinism study.Fish physiology and biochemistry · 2017Article
- Expression Profile Analysis of miR-221 and miR-222 in Different Tissues and Head Kidney Cells of Cynoglossus semilaevis, Following Pathogen Infection.Marine biotechnology (New York, N.Y.) · 2016Article
- Establishment and characterization of an astroglial cell line derived from the brain of half-smooth tongue sole (Cynoglossus semilaevis).Dong wu xue yan jiu = Zoological research · 2015Article
- Establishment and characterization of a gonad cell line from half-smooth tongue sole Cynoglossus semilaevis pseudomale.Fish physiology and biochemistry · 2015Article
- Establishment and characterization of fin cell lines from diploid, triploid, and tetraploid oriental weatherfish (Misgurnus anguillicaudatus).Fish physiology and biochemistry · 2015Article
- Expression profiling analysis of the microRNA response of Cynoglossus semilaevis to Vibrio anguillarum and other stimuli.Marine biotechnology (New York, N.Y.) · 2015Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A new cell line (TSHKC) derived from half-smooth tongue sole (Cynoglossus semilaevis) head kidney was developed. The cell line was subcultured for 40 passages over a period of 360 days. The cell line was optimally maintained in minimum essential medium supplemented with HEPES, antibiotics, fetal bovine serum, 2-Mercaptoethanol (2-Me), sodium pyruvate and basic fibroblast growth factor. The suitable growth temperature for TSHKC cells was 24 °C, and microscopically, TSHKC cells were composed of fibroblast-like cells. Chromosome analysis revealed that the TSHKC cell line had a normal diploid karyotype with 2n = 42, contained the heterogametic W chromosome. The TSHKC cell line was found to be susceptible to lymphocystis disease virus. The fluorescent signals were observed in TSHKC when the cells were transfected with green fluorescent protein and red fluorescent protein reporter plasmids.
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