ArticleApplied and environmental microbiology2012
A specific mutation in the promoter region of the silent cel cluster accounts for the appearance of lactose-utilizing Lactococcus lactis MG1363.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 38 citations in OpenAlex.
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- Disruption of a Transcriptional Repressor by an Insertion Sequence Element Integration Leads to Activation of a Novel Silent Cellobiose Transporter in Lactococcus lactis MG1363.Applied and environmental microbiology · 2017Article
- Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation.BMC genomics · 2017Article
- Transcriptome landscape of Lactococcus lactis reveals many novel RNAs including a small regulatory RNA involved in carbon uptake and metabolism.RNA biology · 2016Article
- Implementation of the agmatine-controlled expression system for inducible gene expression in Lactococcus lactis.Microbial cell factories · 2015Article
- ClaR--a novel key regulator of cellobiose and lactose metabolism in Lactococcus lactis IL1403.Applied microbiology and biotechnology · 2015Article
- Bet-hedging during bacterial diauxic shift.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Zirex: a novel zinc-regulated expression system for Lactococcus lactis.Applied and environmental microbiology · 2013Article
- Next-generation sequencing as an approach to dairy starter selection.Dairy science & technologyReview
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Lactococcus lactis laboratory strain MG1363 has been described to be unable to utilize lactose. However, in a rich medium supplemented with lactose as the sole carbon source, it starts to grow after prolonged incubation periods. Transcriptome analyses showed that L. lactis MG1363 Lac(+) cells expressed celB, encoding a putative cellobiose-specific phosphotransferase system (PTS) IIC component, which is normally silent in MG1363 Lac(-) cells. Nucleotide sequence analysis of the cel cluster of a Lac(+) isolate revealed a change from one of the guanines to adenine in the promoter region. We showed here that one particular mutation, taking place at increased frequency, accounts for the lactose-utilizing phenotype occurring in MG1363 cultures. The G-to-A transition creates a -10 element at an optimal distance from the -35 element. Thus, a fully active promoter is created, allowing transcription of the otherwise cryptic cluster. Nuclear magnetic resonance (NMR) spectroscopy results show that MG1363 Lac(+) uses a novel pathway of lactose utilization.
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