ArticleApplied and environmental microbiology2012
Forward genetic in planta screen for identification of plant-protective traits of Sphingomonas sp. strain Fr1 against Pseudomonas syringae DC3000.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 66 citations in OpenAlex.
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- Structural and functional properties of bacterial communities associated with rootless duckweed (Wolffia globosa) and their effect on the Wolffia growth.Environmental microbiome · 2025Article
- ScAnalyzer: an image processing tool to monitor plant disease symptoms and pathogen spread in Arabidopsis thaliana leaves.Plant methods · 2024Article
- Phyllosphere bacterial community dynamics in response to bacterial wildfire disease: succession and interaction patterns.Frontiers in plant science · 2024Article
- Role of bacterial pathogens in microbial ecological networks in hydroponic plants.Frontiers in plant science · 2024Article
- Contrasting patterns of microbial dominance in theProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Dynamic character displacement among a pair of bacterial phyllosphere commensals in situ.Nature communications · 2022Article
- Responses of the bacterial community of tobacco phyllosphere to summer climate and wildfire disease.Frontiers in plant science · 2022Article
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- Protective role of the Arabidopsis leaf microbiota against a bacterial pathogen.Nature microbiology · 2021Article
- Triterpenoid and Steroidal Saponins Differentially Influence Soil Bacterial Genera.Plants (Basel, Switzerland) · 2021Article
- Conjugation Dynamics of Self-Transmissible and Mobilisable Plasmids intoAntibiotics (Basel, Switzerland) · 2021Article
- A general non-self response as part of plant immunity.Nature plants · 2021Article
- A pattern-triggered immunity-related phenolic, acetosyringone, boosts rapid inhibition of a diverse set of plant pathogenic bacteria.BMC plant biology · 2021Article
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- Microbiota entrapped in recently-formed ice: Paradana Ice Cave, Slovenia.Scientific reports · 2021Article
- Litterbox-A gnotobiotic Zeolite-Clay System to Investigate Arabidopsis-Microbe Interactions.Microorganisms · 2020Article
- Replicating Arabidopsis Model Leaf Surfaces for Phyllosphere Microbiology.Scientific reports · 2019Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sphingomonas sp. strain Fr1 has recently been shown to protect Arabidopsis thaliana against the bacterial leaf pathogen Pseudomonas syringae DC3000. Here, we describe a forward genetic in planta screen to identify genes in Sphingomonas sp. Fr1 necessary for this effect. About 5,000 Sphingomonas sp. Fr1 mini-Tn5 mutants were assayed for a defect in plant protection against a luxCDABE-tagged P. syringae DC3000 derivative in a space-saving 24-well plate system. The bioluminescence of the pathogen was used as the indicator of pathogen proliferation and allowed for the identification of Sphingomonas sp. Fr1 mutants that had lost the ability to restrict pathogen growth before disease symptoms were visible. Potential candidates were validated using the same miniaturized experimental system. Of these mutants, 10 were confirmed as plant protection defective yet colonization competent. The mutants were subsequently evaluated in a previously described standard microbox system, and plants showed enhanced disease phenotypes after pathogen infection relative to those inoculated with the parental strain as a control. However, the disease severities were lower than those observed for control plants that were grown axenically prior to pathogen challenge, which suggests that several traits may contribute to plant protection. Transposon insertion sites of validated mutants with defects in plant protection were determined and mapped to 7 distinct genomic regions. In conclusion, the established screening protocol allowed us to identify mutations that affect plant protection, and it opens the possibility to uncover traits important for in planta microbe-microbe interactions.
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