ArticleApplied and environmental microbiology2012
Involvement of RpoN in regulating bacterial arsenite oxidation.
Article in Applied and environmental microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Unveiling the molecular basis of arsenic resistance in Dietzia maris isolated from arsenic-contaminated soil.BMC biotechnology · 2026Article
- ProPr54 web server: predicting σNAR genomics and bioinformatics · 2025Article
- Biological characterization of Bacillus flexus strain SSAI1 transforming highly toxic arsenite to less toxic arsenate mediated by periplasmic arsenite oxidase enzyme encoded by aioAB genes.Biometals : an international journal on the role of metal ions in biology, biochemistry, and medicine · 2021Article
- Characterization of Arsenite-Oxidizing Bacteria Isolated from Arsenic-Rich Sediments, Atacama Desert, Chile.Microorganisms · 2021Article
- Microbial Oxidation of Arsenite: Regulation, Chemotaxis, Phosphate Metabolism and Energy Generation.Frontiers in microbiology · 2020Review
- Expression of Genes and Proteins Involved in Arsenic Respiration and Resistance in Dissimilatory Arsenate-ReducingApplied and environmental microbiology · 2019Article
- Genetic mechanisms of arsenic detoxification and metabolism in bacteria.Current genetics · 2019Review
- Expression of the arsenite oxidation regulatory operon in Rhizobium sp. str. NT-26 is under the control of two promoters that respond to different environmental cues.MicrobiologyOpen · 2018Article
- An Oxidoreductase AioE is Responsible for Bacterial Arsenite Oxidation and Resistance.Scientific reports · 2017Article
- Identification of N(α)-acetyl-α-lysine as a probable thermolyte and its accumulation mechanism in Salinicoccus halodurans H3B36.Scientific reports · 2015Article
- Arsenite oxidase also functions as an antimonite oxidase.Applied and environmental microbiology · 2015Article
- Regulation of arsenite oxidation by the phosphate two-component system PhoBR in Halomonas sp. HAL1.Frontiers in microbiology · 2015Article
- An ArsR/SmtB family member is involved in the regulation by arsenic of the arsenite oxidase operon in Thiomonas arsenitoxydans.Applied and environmental microbiology · 2014Article
- Organization and regulation of the arsenite oxidase operon of the moderately acidophilic and facultative chemoautotrophic Thiomonas arsenitoxydans.Extremophiles : life under extreme conditions · 2013Article
- Life in an arsenic-containing gold mine: genome and physiology of the autotrophic arsenite-oxidizing bacterium rhizobium sp. NT-26.Genome biology and evolution · 2013Article
- In silico analysis of bacterial arsenic islands reveals remarkable synteny and functional relatedness between arsenate and phosphate.Frontiers in microbiology · 2013Article
- Draft genome sequence of Agrobacterium albertimagni strain AOL15.Journal of bacteriology · 2012Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study with the model organism Agrobacterium tumefaciens, we used a combination of lacZ gene fusions, reverse transcriptase PCR (RT-PCR), and deletion and insertional inactivation mutations to show unambiguously that the alternative sigma factor RpoN participates in the regulation of As(III) oxidation. A deletion mutation that removed the RpoN binding site from the aioBA promoter and an aacC3 (gentamicin resistance) cassette insertional inactivation of the rpoN coding region eliminated aioBA expression and As(III) oxidation, although rpoN expression was not related to cell exposure to As(III). Putative RpoN binding sites were identified throughout the genome and, as examples, included promoters for aioB, phoB1, pstS1, dctA, glnA, glnB, and flgB that were examined by using qualitative RT-PCR and lacZ reporter fusions to assess the relative contribution of RpoN to their transcription. The expressions of aioB and dctA in the wild-type strain were considerably enhanced in cells exposed to As(III), and both genes were silent in the rpoN::aacC3 mutant regardless of As(III). The expression level of glnA was not influenced by As(III) but was reduced (but not silent) in the rpoN::aacC3 mutant and further reduced in the mutant under N starvation conditions. The rpoN::aacC3 mutation had no obvious effect on the expression of glnB, pstS1, phoB1, or flgB. These experiments provide definitive evidence to document the requirement of RpoN for As(III) oxidation but also illustrate that the presence of a consensus RpoN binding site does not necessarily link the associated gene with regulation by As(III) or by this sigma factor.
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