ArticleNature immunology2012
Photocrosslinkable pMHC monomers stain T cells specifically and cause ligand-bound TCRs to be 'preferentially' transported to the cSMAC.
Article in Nature immunology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed, 49 citations in OpenAlex.
- Scalable production of monovalent streptavidin with high yield and purity.Scientific reports · 2026Article
- CD4+T-cells create a stable mechanical environment for force-sensitive TCR:pMHC interactions.Nature communications · 2025Article
- Review
- Monomeric agonist peptide/MHCII complexes activate T-cells in an autonomous fashion.EMBO reports · 2023Article
- A DNA Origami-Based Biointerface to Interrogate the Spatial Requirements for Sensitized T-Cell Antigen Recognition.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Covalent TCR-peptide-MHC interactions induce T cell activation and redirect T cell fate in the thymus.Nature communications · 2022Article
- DNA origami demonstrate the unique stimulatory power of single pMHCs as T cell antigens.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Monomeric TCRs drive T cell antigen recognition.Nature immunology · 2018Article
- Programmed self-assembly of peptide-major histocompatibility complex for antigen-specific immune modulation.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- CD8Current opinion in chemical engineering · 2018Article
- An intermolecular FRET sensor detects the dynamics of T cell receptor clustering.Nature communications · 2017Article
- CD4(+) and CD8(+) TCRβ repertoires possess different potentials to generate extraordinarily high-avidity T cells.Scientific reports · 2016Article
- Monovalent Strep-Tactin for strong and site-specific tethering in nanospectroscopy.Nature nanotechnology · 2016Article
- Action and Traction: Cytoskeletal Control of Receptor Triggering at the Immunological Synapse.Frontiers in immunology · 2016Review
- Measuring TCR-pMHC Binding In Situ using a FRET-based Microscopy Assay.Journal of visualized experiments : JoVE · 2015Article
- F-actin flow drives affinity maturation and spatial organization of LFA-1 at the immunological synapse.The Journal of cell biology · 2015Article
- The dendritic cell cytoskeleton promotes T cell adhesion and activation by constraining ICAM-1 mobility.The Journal of cell biology · 2015Article
- Molecular architecture of the αβ T cell receptor-CD3 complex.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Force and affinity in ligand discrimination by the TCR.Trends in immunology · 2014Review
- Insights into the initiation of TCR signaling.Nature immunology · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The binding of T cell antigen receptors (TCRs) to specific complexes of peptide and major histocompatibility complex (pMHC) is typically of very low affinity, which necessitates the use of multimeric pMHC complexes to label T lymphocytes stably. We report here the development of pMHC complexes able to be crosslinked by ultraviolet irradiation; even as monomers, these efficiently and specifically stained cognate T cells. We also used this reagent to probe T cell activation and found that a covalently bound pMHC was more stimulatory than an agonist pMHC on lipid bilayers. This finding suggested that serial engagement of TCRs is dispensable for activation when a substantial fraction of TCRs are stably engaged. Finally, pMHC-bound TCRs were 'preferentially' transported into the central supramolecular activation cluster after activation, which suggested that ligand engagement enabled linkage of the TCR and its associated CD3 signaling molecules to the cytoskeleton.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.