ArticleDevelopmental cell2012
Optical tweezers studies on Notch: single-molecule interaction strength is independent of ligand endocytosis.
Article in Developmental cell, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
39 citing papers in PubMed.
- Article
- Article
- Spatio-Temporal Regulation of Notch Activation in Asymmetrically Dividing Sensory Organ Precursor Cells inCells · 2024Review
- Investigation of Soft Matter Nanomechanics by Atomic Force Microscopy and Optical Tweezers: A Comprehensive Review.Nanomaterials (Basel, Switzerland) · 2023Review
- Trafficking in blood vessel development.Angiogenesis · 2022Review
- Simulation of receptor triggering by kinetic segregation shows role of oligomers and close contacts.Biophysical journal · 2022Article
- Nano-Precision Tweezers for Mechanosensitive Proteins and Beyond.Molecules and cells · 2022Review
- Par3 cooperates with Sanpodo for the assembly of Notch clusters following asymmetric division ofeLife · 2021Article
- Biophysics of Notch Signaling.Annual review of biophysics · 2021Review
- The Clathrin adaptor AP-1 and Stratum act in parallel pathways to control Notch activation inDevelopment (Cambridge, England) · 2021Article
- Probing Ligand-Receptor Interaction in Living Cells Using Force Measurements With Optical Tweezers.Frontiers in bioengineering and biotechnology · 2020Article
- Probing force in living cells with optical tweezers: from single-molecule mechanics to cell mechanotransduction.Biophysical reviews · 2019Review
- A role for actomyosin contractility in Notch signaling.BMC biology · 2019Article
- Mechanosensitivity of Jagged-Notch signaling can induce a switch-type behavior in vascular homeostasis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Mammalian Numb protein antagonizes Notch by controlling postendocytic trafficking of the Notch ligand Delta-like 4.The Journal of biological chemistry · 2017Article
- Article
- Selective regulation of Notch ligands during angiogenesis is mediated by vimentin.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The Canonical Notch Signaling Pathway: Structural and Biochemical Insights into Shape, Sugar, and Force.Developmental cell · 2017Review
- Intercellular Tension Negatively Regulates Angiogenic Sprouting of Endothelial Tip Cells via Notch1-Dll4 Signaling.Advanced biosystems · 2017Article
- Cilia Control Vascular Mural Cell Recruitment in Vertebrates.Cell reports · 2017Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Notch signaling controls diverse cellular processes critical to development and disease. Cell surface ligands bind Notch on neighboring cells but require endocytosis to activate signaling. The role ligand endocytosis plays in Notch activation has not been established. Here we integrate optical tweezers with cell biological and biochemical methods to test the prevailing model that ligand endocytosis facilitates recycling to enhance ligand interactions with Notch necessary to trigger signaling. Specifically, single-molecule measurements indicate that interference of ligand endocytosis and/or recycling does not alter the force required to rupture bonds formed between cells expressing the Notch ligand Delta-like1 (Dll1) and laser-trapped Notch1 beads. Together, our analyses eliminate roles for ligand endocytosis and recycling in Dll1-Notch1 interactions and indicate that recycling indirectly affects signaling by regulating the accumulation of cell surface ligand. Importantly, our study demonstrates the utility of optical tweezers to test a role for ligand endocytosis in generating cell-mediated mechanical force.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.