ArticleCurrent biology : CB2012
The budding yeast nuclear envelope adjacent to the nucleolus serves as a membrane sink during mitotic delay.
Article in Current biology : CB, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.
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Who cites it
57 citing papers in PubMed, 93 citations in OpenAlex.
- Chromosome Segregation in Closed Mitosis Under an Excess of Nuclear Envelope.Biology of the cell · 2025Article
- The partitioning of fatty acids between membrane and storage lipids controls ER membrane expansion.bioRxiv : the preprint server for biology · 2024Article
- Article
- A high-content screen reveals new regulators of nuclear membrane stability.Scientific reports · 2024Article
- A high-content screen reveals new regulators of nuclear membrane stability.bioRxiv : the preprint server for biology · 2023Article
- SUMOylation at the inner nuclear membrane facilitates nuclear envelope biogenesis during mitosis.The Journal of cell biology · 2023Article
- SIR telomere silencing depends on nuclear envelope lipids and modulates sensitivity to a lysolipid.The Journal of cell biology · 2023Article
- A Yeast Mitotic Tale for the Nucleus and the Vacuoles to Embrace.International journal of molecular sciences · 2023Review
- Distinct domains in Ndc1 mediate its interaction with the Nup84 complex and the nuclear membrane.The Journal of cell biology · 2023Article
- One-carbon metabolic enzymes are regulated during cell division and make distinct contributions to the metabolome and cell cycle progression in Saccharomyces cerevisiae.G3 (Bethesda, Md.) · 2023Article
- Review
- Diacylglycerol at the inner nuclear membrane fuels nuclear envelope expansion in closed mitosis.Journal of cell science · 2023Article
- The vacuole shapes the nucleus and the ribosomal DNA loop during mitotic delays.Life science alliance · 2022Article
- Nuclear ingression of cytoplasmic bodies accompanies a boost in autophagy.Life science alliance · 2022Article
- Phenomics approaches to understand genetic networks and gene function in yeast.Biochemical Society transactions · 2022Article
- Article
- Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.Developmental cell · 2021Article
- Cell biology: How does the nucleus get its membrane?Current biology : CB · 2021Article
- The challenge of staying in shape: nuclear size matters.Current genetics · 2021Review
- Oxidative agents elicit endoplasmic reticulum morphological changes suggestive of alterations in lipid metabolism.microPublication biology · 2021Article
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11 authors at 3 institutions in 2 countries.
Funding
Abstract
The mechanisms that dictate nuclear shape are largely unknown. Here we screened the budding yeast deletion collection for mutants with abnormal nuclear shape. A common phenotype was the appearance of a nuclear extension, particularly in mutants in DNA repair and chromosome segregation genes. Our data suggest that these mutations led to the abnormal nuclear morphology indirectly, by causing a checkpoint-induced cell-cycle delay. Indeed, delaying cells in mitosis by other means also led to the appearance of nuclear extensions, whereas inactivating the DNA damage checkpoint pathway in a DNA repair mutant reduced the fraction of cells with nuclear extensions. Formation of a nuclear extension was specific to a mitotic delay, because cells arrested in S or G2 had round nuclei. Moreover, the nuclear extension always coincided with the nucleolus, while the morphology of the DNA mass remained largely unchanged. Finally, we found that phospholipid synthesis continued unperturbed when cells delayed in mitosis, and inhibiting phospholipid synthesis abolished the formation of nuclear extensions. Our data suggest a mechanism that promotes nuclear envelope expansion during mitosis. When mitotic progression is delayed, cells sequester the added membrane to the nuclear envelope associated with the nucleolus, possibly to avoid disruption of intranuclear organization.
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