ArticleAnalytical chemistry2012
Separation of cis-trans phospholipid isomers using reversed phase LC with high resolution MS detection.
Article in Analytical chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 64 citations in OpenAlex.
- LC-ESI-HRMS - lipidomics of phospholipids : Characterization of extraction, chromatography and detection parameters.Analytical and bioanalytical chemistry · 2024Article
- Age-related changes in circadian regulation of the human plasma lipidome.Communications biology · 2023Article
- Characterization of lipid carbon-carbon double-bond isomerism via ion mobility-mass spectrometry (IMS-MS) combined with cuprous ion-induced fragmentation.International journal of mass spectrometry · 2022Article
- Coenzyme Q10 in the eye isomerizes by sunlight irradiation.Scientific reports · 2022Article
- Dual-resolving of positional and geometric isomers of C=C bonds via bifunctional photocycloaddition-photoisomerization reaction system.Nature communications · 2022Article
- Lipidomics in Biomarker Research.Handbook of experimental pharmacology · 2022Review
- Role of lipidomics in assessing the functional lipid composition in breast milk.Frontiers in nutrition · 2022Review
- Revealing Fatty Acid Heterogeneity in Staphylococcal Lipids with Isotope Labeling and RPLC-IM-MS.Journal of the American Society for Mass Spectrometry · 2021Article
- Multi-Omics Reveals Impact of Cysteine Feed Concentration and Resulting Redox Imbalance on Cellular Energy Metabolism and Specific Productivity in CHO Cell Bioprocessing.Biotechnology journal · 2020Article
- Targeting Modified Lipids during Routine Lipidomics Analysis using HILIC and C30 Reverse Phase Liquid Chromatography coupled to Mass Spectrometry.Scientific reports · 2019Article
- Optimization of Electrospray Ionization Source Parameters for Lipidomics To Reduce Misannotation of In-Source Fragments as Precursor Ions.Analytical chemistry · 2018Article
- Pinpointing Double Bond and sn-Positions in Glycerophospholipids via Hybrid 193 nm Ultraviolet Photodissociation (UVPD) Mass Spectrometry.Journal of the American Chemical Society · 2017Article
- Off-line mixed-mode liquid chromatography coupled with reversed phase high performance liquid chromatography-high resolution mass spectrometry to improve coverage in lipidomics analysis.Analytica chimica acta · 2017Article
- LipidFrag: Improving reliability of in silico fragmentation of lipids and application to the Caenorhabditis elegans lipidome.PloS one · 2017Article
- Comprehensive untargeted lipidomic analysis using core-shell C30 particle column and high field orbitrap mass spectrometer.Journal of chromatography. A · 2016Article
- Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry.The Analyst · 2016Article
- Lipidomic analysis of cerebrospinal fluid by mass spectrometry-based methods.Journal of inherited metabolic disease · 2015Review
- Enhanced lipid isomer separation in human plasma using reversed-phase UPLC with ion-mobility/high-resolution MS detection.Journal of lipid research · 2014Article
- Dietary macronutrients modulate the fatty acyl composition of rat liver mitochondrial cardiolipins.Journal of lipid research · 2013Article
- Annotation of the human cerebrospinal fluid lipidome using high resolution mass spectrometry and a dedicated data processing workflow.Metabolomics : Official journal of the Metabolomic SocietyArticle
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The increased presence of synthetic trans fatty acids into western diets has been shown to have deleterious effects on physiology and raising an individual's risk of developing metabolic disease, cardiovascular disease, and stroke. The importance of these fatty acids for health and the diversity of their (patho) physiological effects suggest that not only should the free trans fatty acids be studied but also monitoring the presence of these fats into the side chains of biological lipids, such as glycerophospholipids, is also essential. We developed a high resolution LC-MS method that quantitatively monitors the major lipid classes found in biospecimens in an efficient, sensitive, and robust manner while also characterizing individual lipid side chains through the use of high energy collisional dissociation (HCD) fragmentation and chromatographic alignment. We herein show how this previously described reversed phase method can baseline separate the cis-trans isomers of phosphatidylglycerol and phosphatidylcholine (PC) with two 18:1 side chains, in both positive and negative mode, as neat solutions and when spiked into a biological matrix. Endogenous PC (18:1/18:1)-cis and PC (18:1/18:1)-trans isomers were examined in mitochondrial and serum profiling studies, where rats were fed diets enriched in either trans 18:1 fatty acids or cis 18:1 fatty acids. In this study, we determined the cis:trans isomer ratios of PC (18:1/18:1) and related this ratio to dietary composition. This generalized LC-MS method enables the monitoring of trans fats in biological lipids in the context of a nontargeted method, allowing for relative quantitation and enhanced identification of unknown lipids in complex matrixes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.