ArticleTissue engineering. Part A2012
Cotransplantation of adipose-derived mesenchymal stromal cells and endothelial cells in a modular construct drives vascularization in SCID/bg mice.
Article in Tissue engineering. Part A, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Adipose Tissue Engineering Biomaterials: Smart Scaffolds, Vascularization, and Clinical Frontiers.Biomolecules · 2026Review
- Pre-cultured, cell-encapsulating fibrin microbeads for the vascularization of ischemic tissues.Journal of biomedical materials research. Part A · 2024Article
- Review
- Vascular reconstruction of the decellularized biomatrix for whole-organ engineering-a critical perspective and future strategies.Frontiers in bioengineering and biotechnology · 2023Review
- Bioengineering the Vascularized Endocrine Pancreas: A Fine-Tuned Interplay Between Vascularization, Extracellular-Matrix-Based Scaffold Architecture, and Insulin-Producing Cells.Transplant international : official journal of the European Society for Organ Transplantation · 2022Review
- Engineering the vasculature for islet transplantation.Acta biomaterialia · 2019Review
- Injectable Biomaterials in Plastic and Reconstructive Surgery: A Review of the Current Status.Tissue engineering and regenerative medicine · 2018Review
- Modular tissue engineering for the vascularization of subcutaneously transplanted pancreatic islets.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Transplantation of bioengineered rat lungs recellularized with endothelial and adipose-derived stromal cells.Scientific reports · 2017Article
- Growth Factor Content in Human Sera Affects the Isolation of Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow.Frontiers in cell and developmental biology · 2016Article
- A novel high-speed production process to create modular components for the bottom-up assembly of large-scale tissue-engineered constructs.Advanced healthcare materials · 2015Article
- Vasculogenesis and Angiogenesis in Modular Collagen-Fibrin Microtissues.Biomaterials science · 2014Article
- Strategies for directing the structure and function of three-dimensional collagen biomaterials across length scales.Acta biomaterialia · 2014Review
- Del-1 overexpression in endothelial cells increases vascular density in tissue-engineered implants containing endothelial cells and adipose-derived mesenchymal stromal cells.Tissue engineering. Part A · 2014Article
- Using Del-1 to tip the angiogenic balance in endothelial cells in modular constructs.Tissue engineering. Part A · 2014Article
- SLIT3-ROBO4 activation promotes vascular network formation in human engineered tissue and angiogenesis in vivo.Journal of molecular and cellular cardiology · 2013Article
- Directing the assembly of spatially organized multicomponent tissues from the bottom up.Trends in cell biology · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A modular approach to adipose tissue engineering was explored by embedding adipose-derived mesenchymal stromal cells (adMSC) in sub-mm-sized collagen rods or "modules" and coating with human microvascular endothelial cells (HMEC). After subcutaneous injection into a SCID/Bg mouse, HMEC on modules containing embedded adMSC appeared to detach from the modules to form vessels as early as day 3, as confirmed by the human EC-specific UEA-1 lectin stain, and these vessels persisted for up to 90 days. Vessel numbers decreased over 14 days, but vessel size increased suggesting a maturing of the vasculature. Vessel perfusion with the host was confirmed at 21 days by microCT. HMEC on modules without embedded adMSC remained attached to the module surface at day 3 and UEA-1 staining disappeared over 14 days suggesting cell death. It appeared that cotransplantation with adMSC had an anti-apoptotic and proangiogenic effect on HMEC. The early revascularization strategy may be successful in supporting adMSC viability and differentiation, as a preliminary study suggests progressive fat accumulation in the HMEC+adMSC implants: ∼60% of the implant area stained positive for Oil Red O by day 90. adMSC-embedded modules without HMEC surface coating did not show similar levels of Oil Red O staining. All implant volumes decreased over the time course of the experiment, yet HMEC+adMSC module implants were larger than adMSC-only implants at day 90. Collagen gel is mechanically weak and contracts in vivo making it unsuitable as a biomaterial for adipose tissue engineering where volume maintenance is critical. When combined with an appropriate biomaterial, the modular approach to adipose tissue engineering may represent a successful strategy to engineer soft tissue substitutes of clinical relevance.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.