ArticleJournal of oncology2012
Fluorescence In Situ Hybridization for MicroRNA Detection in Archived Oral Cancer Tissues.
Article in Journal of oncology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Chemically Modified DNAzyme with Enhanced Activity for Sensitive MicroRNA Imaging in Live Cells.Molecules (Basel, Switzerland) · 2026Article
- Role of MicroRNA-502-3p in Human Diseases.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Methylation-associated silencing of miR-9-1 promotes nasopharyngeal carcinoma progression and glycolysis via HK2.Cancer science · 2021Article
- MicroRNA-4516 suppresses pancreatic cancer development via negatively regulating orthodenticle homeobox 1.International journal of biological sciences · 2020Article
- LINC02418 promotes colon cancer progression by suppressing apoptosis via interaction with miR-34b-5p/BCL2 axis.Cancer cell international · 2020Article
- miR-137-3p Modulates the Progression of Prostate Cancer by Regulating the JNK3/EZH2 Axis.OncoTargets and therapy · 2020Article
- Electrochemical detection of urinary microRNAs via sulfonamide-bound antisense hybridisation.Sensors and actuators. B, Chemical · 2017Article
- miR-30c and semaphorin 3A determine adult neurogenesis by regulating proliferation and differentiation of stem cells in the subventricular zones of mouse.Cell proliferation · 2016Article
- Decrease of miR-146a is associated with the aggressiveness of human oral squamous cell carcinoma.Archives of oral biology · 2015Article
- Small RNA Detection by in Situ Hybridization Methods.International journal of molecular sciences · 2015Review
- Article
- Clinical implications of miRNAs in the pathogenesis, diagnosis and therapy of pancreatic cancer.Advanced drug delivery reviews · 2015Review
- An ion-exchange nanomembrane sensor for detection of nucleic acids using a surface charge inversion phenomenon.Biosensors & bioelectronics · 2014Article
- Combined fluorescent in situ hybridization for detection of microRNAs and immunofluorescent labeling for cell-type markers.Frontiers in cellular neuroscience · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The noncoding RNA designated as microRNA (miRNA) is a large group of small single-stranded regulatory RNA and has generated wide-spread interest in human disease studies. To facilitate delineating the role of microRNAs in cancer pathology, we sought to explore the feasibility of detecting microRNA expression in formalin-fixed paraffin-embedded (FFPE) tissues. Using FFPE materials, we have compared fluorescent in situ hybridization (FISH) procedures to detect miR-146a with (a) different synthetic probes: regular custom DNA oligonucleotides versus locked nucleic acid (LNA) incorporated DNA oligonucleotides; (b) different reporters for the probes: biotin versus digoxigenin (DIG); (c) different visualization: traditional versus tyramide signal amplification (TSA) system; (d) different blocking reagents for endogenous peroxidase. Finally, we performed miR-146a FISH on a commercially available oral cancer tissue microarray, which contains 40 cases of oral squamous cell carcinoma (OSCC) and 10 cases of normal epithelia from the human oral cavity. A sample FISH protocol for detecting miR-146a is provided. In summary, we have established reliable in situ hybridization procedures for detecting the expression of microRNA in FFPE oral cancer tissues. This method is an important tool for studies on the involvement of microRNA in oral cancer pathology and may have potential prognostic or diagnostic value.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.