ArticleThe Journal of biological chemistry2012
Hyaluronan expressed by the hematopoietic microenvironment is required for bone marrow hematopoiesis.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Exploring MSC and HSPC interactions: new frontiers in hematopoiesis and transplant medicine.Stem cell research & therapy · 2025Review
- Platelet Hyaluronan Synthase 3 Regulates Thrombin Signaling and Adhesion to Fibrinogen Under Venous Shear.Proteoglycan research · 2025Article
- 4-Methylumbeliferone Treatment at a Dose of 1.2 g/kg/Day Is Safe for Long-Term Usage in Rats.International journal of molecular sciences · 2023Article
- Hyaluronic acid-GPRC5C signalling promotes dormancy in haematopoietic stem cells.Nature cell biology · 2022Article
- Oral treatment of 4-methylumbelliferone reduced perineuronal nets and improved recognition memory in mice.Brain research bulletin · 2022Article
- The extracellular matrix of hematopoietic stem cell niches.Advanced drug delivery reviews · 2022Review
- Bone marrow mesenchymal stromal cell-derived extracellular matrix displays altered glycosaminoglycan structure and impaired functionality in Myelodysplastic Syndromes.Frontiers in oncology · 2022Article
- An Overview of Different Strategies to Recreate the Physiological Environment in Experimental Erythropoiesis.International journal of molecular sciences · 2020Review
- Remodelling of the bone marrow microenvironment by stromal hyaluronan modulates the malignancy of breast cancer cells.Cell communication and signaling : CCS · 2020Article
- Human induced pluripotent stem cell-derived mesenchymal stem cells promote healing via TNF-α-stimulated gene-6 in inflammatory bowel disease models.Cell death & disease · 2019Article
- Hyaluronan abrogates imatinib-induced senescence in chronic myeloid leukemia cell lines.Scientific reports · 2019Article
- Hyaluronan in inflammatory bowel disease: Cross-linking inflammation and coagulation.Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- Hyaluronan biology: A complex balancing act of structure, function, location and context.Matrix biology : journal of the International Society for Matrix Biology · 2019Review
- 3D models of the bone marrow in health and disease: yesterday, today and tomorrow.MRS communications · 2019Article
- The NOTCH1/CD44 axis drives pathogenesis in a T cell acute lymphoblastic leukemia model.The Journal of clinical investigation · 2018Article
- The role of extracellular matrix stiffness in megakaryocyte and platelet development and function.American journal of hematology · 2018Review
- CD44-mediated hyaluronan binding marks proliferating hematopoietic progenitor cells and promotes bone marrow engraftment.PloS one · 2018Article
- Article
- 4-methylumbelliferone and imatinib combination enhances senescence induction in chronic myeloid leukemia cell lines.Investigational new drugs · 2017Article
- Hyaluronan as a therapeutic target in human diseases.Advanced drug delivery reviews · 2016Review
Corrections and comments
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Authors and funding
12 authors at 7 institutions in 4 countries.
Funding
Abstract
The contribution of hyaluronan (HA) to the regulatory network of the hematopoietic microenvironment was studied using knock-out mice of three hyaluronan synthase genes (Has1, Has2, and Has3). The number of hematopoietic progenitors was decreased in bone marrow and increased in extramedullary sites of Prx1-Cre;Has2(flox/flox);Has1(-/-);Has3(-/-) triple knock-out (tKO) mice as compared with wild type (WT) and Has1(-/-);Has3(-/-) double knock-out (dKO) mice. In line with this observation, decreased hematopoietic activity was observed in long term bone marrow cultures (LTBMC) from tKO mice, whereas the formation of the adherent layer and generation of hematopoietic cells in WT and dKO cultures was not different. 4-Methylumbelliferone (4MU) was used to pharmacologically inhibit the production of HA in LTBMC. Treatment with 4MU inhibited HA synthesis, decreased expression of HAS2 and HAS3, and eliminated hematopoiesis in LTBMC, and this effect was alleviated by the addition of exogenous HA. Exogenous HA also augmented the cell motility in LTBMC, which correlated with the HA-stimulated production of chemokines and growth factors. Conditioned media from HA-induced LTBMC enhanced the chemotaxis of hematopoietic stem/progenitor cells (HSPC) in response to SDF-1. Exposure of endothelial cells to 4MU decreased their ability to support HSPC rolling and adhesion. In addition, migration of transplanted HSPC into the marrow of 4MU-pretreated mice was lower than in untreated mice. Collectively, the results suggest that HA depletion reduces the ability of the microenvironment to support HSPC, and confirm a role for HA as a necessary regulatory element in the structure of the hematopoietic microenvironment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.