ArticleThe Journal of biological chemistry2012
L-xylo-3-hexulose reductase is the missing link in the oxidoreductive pathway for D-galactose catabolism in filamentous fungi.
Article in The Journal of biological chemistry, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed, 42 citations in OpenAlex.
- Model-driven exploration of lactose and galactose metabolism via an oxidoreductive pathway in Sungouiella intermedia for cell factory applications.Microbial cell factories · 2026Article
- The genome-scale sugar metabolic model fromCurrent research in microbial sciences · 2026Article
- L-Rhamnose Dehydrogenase LraA ofJournal of fungi (Basel, Switzerland) · 2025Article
- Comparative transcriptome analyses and CRISPR/Cas9-mediated functional study ofFrontiers in microbiology · 2025Article
- Metabolic engineering reveals the relative importance of different sugar catabolic pathways during consumption of plant biomass byCurrent research in microbial sciences · 2025Article
- A unique metabolic gene cluster regulates lactose and galactose metabolism in the yeastApplied and environmental microbiology · 2024Article
- Studying microbial triglyceride production from corn stover saccharides unveils insights into the galactose metabolism of Ustilago maydis.Microbial cell factories · 2024Article
- Transcriptomic and metabolic changes in Trichoderma reesei caused by mutation in xylanase regulator 1 (xyr1).Biotechnology for biofuels and bioproducts · 2024Article
- Unveiling a Microexon Switch: Novel Regulation of the Activities of Sugar Assimilation and Plant-Cell-Wall-Degrading Xylanases and Cellulases by Xlr2 inInternational journal of molecular sciences · 2024Article
- The Sugar Metabolic Model ofJournal of fungi (Basel, Switzerland) · 2022Article
- GalR, GalX and AraR co-regulate d-galactose and l-arabinose utilization in Aspergillus nidulans.Microbial biotechnology · 2022Article
- Transcriptional Profiling ofFrontiers in microbiology · 2021Article
- The regulatory and transcriptional landscape associated with carbon utilization in a filamentous fungus.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- In silico evolution ofBiotechnology for biofuels · 2020Article
- Evolution and comparative genomics of the most common Trichoderma species.BMC genomics · 2019Article
- Proteolytic analysis of Trichoderma reesei in celluase-inducing condition reveals a role for trichodermapepsin (TrAsP) in cellulase production.Journal of industrial microbiology & biotechnology · 2019Article
- The gold-standard genome ofStudies in mycology · 2018Article
- Alternative Substrate Metabolism inFrontiers in microbiology · 2018Review
- Article
- Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica.Biotechnology for biofuels · 2015Article
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In addition to the well established Leloir pathway for the catabolism of d-galactose in fungi, the oxidoreductive pathway has been recently identified. In this oxidoreductive pathway, D-galactose is converted via a series of NADPH-dependent reductions and NAD(+)-dependent oxidations into D-fructose. The pathway intermediates include galactitol, L-xylo-3-hexulose, and d-sorbitol. This study identified the missing link in the pathway, the L-xylo-3-hexulose reductase that catalyzes the conversion of L-xylo-3-hexulose to D-sorbitol. In Trichoderma reesei (Hypocrea jecorina) and Aspergillus niger, we identified the genes lxr4 and xhrA, respectively, that encode the l-xylo-3-hexulose reductases. The deletion of these genes resulted in no growth on galactitol and in reduced growth on D-galactose. The LXR4 was heterologously expressed, and the purified protein showed high specificity for L-xylo-3-hexulose with a K(m) = 2.0 ± 0.5 mm and a V(max) = 5.5 ± 1.0 units/mg. We also confirmed that the product of the LXR4 reaction is D-sorbitol.
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