ArticleNature protocols2012
Targeted axon-attached recording with fluorescent patch-clamp pipettes in brain slices.
Article in Nature protocols, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
31 citing papers in PubMed, 55 citations in OpenAlex.
- In Vitro Reconstruction of Axonal Heat Sensing with a Photothermal Nerve-on-a-Chip.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Mechanical force locally damages, remodels, and stabilizes the lattice of spindle microtubules.Current biology : CB · 2026Article
- Mammalian metaphase kinetochores are elastic and require condensin for robust structure and function.bioRxiv : the preprint server for biology · 2025Article
- Constructive Neuroengineering of Axon Polarization Control Using Modifiable Agarose Gel Platforms for Neuronal Circuit Construction.Gels (Basel, Switzerland) · 2025Article
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.Current biology : CB · 2025Article
- SKAP binding to microtubules reduces friction at the kinetochore-microtubule interface and increases attachment stability under force.bioRxiv : the preprint server for biology · 2024Article
- Clustered synapses develop in distinct dendritic domains in visual cortex before eye opening.eLife · 2024Article
- Beyond a Transmission Cable-New Technologies to Reveal the Richness in Axonal Electrophysiology.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Review
- Measuring Action Potential Propagation Velocity in Murine Cortical Axons.Bio-protocol · 2023Article
- Optimized Protocol for Proportionate CNS Cell Retrieval as a Versatile Platform for Cellular and Molecular Phenomapping in Aging and Neurodegeneration.International journal of molecular sciences · 2022Article
- Recording Saltatory Conduction Along Sensory Axons Using a High-Density Microelectrode Array.Frontiers in neuroscience · 2022Article
- Voltage-Gated Potassium Channels Ensure Action Potential Shape Fidelity in Distal Axons.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- In Vivo Whole-Cell Patch-Clamp Methods: Recent Technical Progress and Future Perspectives.Sensors (Basel, Switzerland) · 2021Review
- TbScience advances · 2021Article
- Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structure.The Journal of cell biology · 2020Article
- Article
- Approaches and Limitations in the Investigation of Synaptic Transmission and Plasticity.Frontiers in synaptic neuroscience · 2019Article
- Technologies to Study Action Potential Propagation With a Focus on HD-MEAs.Frontiers in cellular neuroscience · 2019Article
- A Versatile and Open-Source Rapid LED Switching System for One-Photon Imaging and Photo-Activation.Frontiers in cellular neuroscience · 2018Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Understanding the physiology of axons in the central nervous system requires experimental access to intact axons. This protocol describes how to perform cell-attached recordings from narrow axon fibers (ϕ <1 μm) in acute and cultured brain slice preparations (with a success rate of ∼50%). By using fluorophore-coated glass pipettes and Nipkow disk confocal microscopy, fluorescently labeled axons can be visually targeted under online optical control. In the cell-attached configuration, axonal action potentials are extracellularly recorded as unit-like, sharp negative currents. The axon morphology labeling and cell-attached recordings of axons can be completed within 1-2 h. The recordings are stable for at least 30 min.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.