ArticleVirus research2012
Core protein-mediated 5'-3' annealing of the West Nile virus genomic RNA in vitro.
Article in Virus research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Conserved long-range interactions are required for stable folding of orthoflaviviral genomic RNA.Nucleic acids research · 2025Article
- Detailed in silico Evaluation of WNV Proteins: Dynamic and Thermodynamic Insights into Doravirine as a Potential Multitarget Agent.Drug design, development and therapy · 2025Article
- The Pseudo-Circular Genomes of Flaviviruses: Structures, Mechanisms, and Functions of Circularization.Cells · 2021Review
- Dengue and Zika Viruses: Epidemiological History, Potential Therapies, and Promising Vaccines.Tropical medicine and infectious disease · 2020Review
- The flavivirus polymerase NS5 regulates translation of viral genomic RNA.Nucleic acids research · 2020Article
- Universal RNAFrontiers in microbiology · 2020Review
- Structural and Functional Properties of the Capsid Protein of Dengue and RelatedInternational journal of molecular sciences · 2019Article
- Disordered RNA chaperones can enhance nucleic acid folding via local charge screening.Nature communications · 2019Article
- Structure mapping of dengue and Zika viruses reveals functional long-range interactions.Nature communications · 2019Article
- The Host Factor AUF1 p45 Supports Flavivirus Propagation by Triggering the RNA Switch Required for Viral Genome Cyclization.Journal of virology · 2018Article
- The Multiples Fates of the Flavivirus RNA Genome During Pathogenesis.Frontiers in genetics · 2018Review
- Properties and Functions of the Dengue Virus Capsid Protein.Annual review of virology · 2016Review
- Functions of the 3' and 5' genome RNA regions of members of the genus Flavivirus.Virus research · 2015Review
- AUF1 p45 promotes West Nile virus replication by an RNA chaperone activity that supports cyclization of the viral genome.Journal of virology · 2014Article
- Functional long-range RNA-RNA interactions in positive-strand RNA viruses.Nature reviews. Microbiology · 2014Review
- Amodiaquine, an antimalarial drug, inhibits dengue virus type 2 replication and infectivity.Antiviral research · 2014Article
- Cellular DDX3 regulates Japanese encephalitis virus replication by interacting with viral un-translated regions.Virology · 2014Article
- Activation of 2' 5'-oligoadenylate synthetase by stem loops at the 5'-end of the West Nile virus genome.PloS one · 2014Article
- Review
- Nucleolin interacts with the dengue virus capsid protein and plays a role in formation of infectious virus particles.Journal of virology · 2013Article
Corrections and comments
- Reprinted as
Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome cyclization through conserved RNA sequences located in the 5' and 3' terminal regions of flavivirus genomic RNA is essential for virus replication. Although the role of various cis-acting RNA elements in panhandle formation is well characterized, almost nothing is known about the potential contribution of protein cofactors to viral RNA cyclization. Proteins with nucleic acid chaperone activities are encoded by many viruses (e.g., retroviruses, coronaviruses) to facilitate RNA structural rearrangements and RNA-RNA interactions during the viral replicative cycle. Since the core protein of flaviviruses is also endowed with potent RNA chaperone activities, we decided to examine the effect of West Nile virus (WNV) core on 5'-3' genomic RNA annealing in vitro. Core protein binding resulted in a dramatic, dose-dependent increase in 5'-3' complex formation. Mutations introduced in either the UAR (upstream AUG region) or CS (conserved sequence) elements of the viral RNA diminished core protein-dependent annealing, while compensatory mutations restored the 5'-3' RNA interaction. The activity responsible for stimulating RNA annealing was mapped to the C-terminal RNA-binding region of WNV core protein. These results indicate that core protein - besides its function in viral particle formation - might be involved in the regulation of flavivirus genomic RNA cyclization, and thus virus replication.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.