ArticleAmerican journal of physiology. Cell physiology2012
VDR and CYP27B1 are expressed in C2C12 cells and regenerating skeletal muscle: potential role in suppression of myoblast proliferation.
Article in American journal of physiology. Cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 85 papers, 1 of them a synthesis that pooled it.
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Who cites it
85 citing papers in PubMed, 1 synthesis or guideline pooled it.
- The Role of Vitamin D in Skeletal Muscle Repair and Regeneration in Animal Models and Humans: A Systematic Review.Nutrients · 2023Pooled it
- No effect of 25-hydroxyvitamin D supplementation on the skeletal muscle transcriptome in vitamin D deficient frail older adults.BMC geriatrics · 2019Trial
- Trial
- Effects of 1,25-dihydroxyvitamin D3 and vitamin D3 on the expression of the vitamin d receptor in human skeletal muscle cells.Calcified tissue international · 2015Trial
- Vitamin D supplementation does not improve human skeletal muscle contractile properties in insufficient young males.European journal of applied physiology · 2014Trial
- A randomized study on the effect of vitamin D₃ supplementation on skeletal muscle morphology and vitamin D receptor concentration in older women.The Journal of clinical endocrinology and metabolism · 2013Trial
- Higher serum 25-hydroxyvitamin D concentrations associate with a faster recovery of skeletal muscle strength after muscular injury.Nutrients · 2013Trial
- Observational
- Vitamin D Status and Supplementation and the Functional Outcomes of Human Musculoskeletal Tissues: A Stratified Systematic Review.Health science reports · 2026Review
- Targeting nuclear receptors in muscular dystrophies and regenerative myogenesis.Frontiers in cell and developmental biology · 2026Review
- Regulatory role of vitamin DScientific reports · 2025Article
- Pathophysiological Role of Vitamin D Deficiency in Down Syndrome: Insights into Metabolic Dysfunction and Sarcopenia.International journal of molecular sciences · 2025Review
- Stem Cell Aging and Rejuvenation in the Skeletal Muscle System.Rejuvenation research · 2025Review
- Single-Nucleotide Polymorphisms (SNPs) in Vitamin D Physiology Genes May Modulate Serum 25(OH)D Levels in Well-Trained CrossFitInternational journal of molecular sciences · 2025Observational
- The Importance of Vitamin D and Magnesium in Athletes.Nutrients · 2025Review
- Vitamin D stimulates Il-15 synthesis in rodent muscle.Biochemistry and biophysics reports · 2025Article
- Effect of serum 25-hydroxyvitamin D level on quadriceps strength: a systematic review and meta-analysis.BMC sports science, medicine & rehabilitation · 2024Article
- Evaluation of Nutrition and Performance Parameters in Division 1 Collegiate Athletes.Nutrients · 2024Article
- Effects of Vitamin D Supplementation in Elite Athletes: A Systematic Review.Orthopaedic journal of sports medicine · 2024Review
- Vitamin D status associates with skeletal muscle loss after anterior cruciate ligament reconstruction.JCI insight · 2023Article
25 more citing papers are in PubMed but not listed here.
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Authors and funding
4 authors.
Funding
Abstract
1α,25(OH)(2)D(3), the active form of vitamin D(3), has been reported to regulate the cell biology of skeletal muscle. However, there has been some controversy about the expression of the vitamin D receptor (VDR) and thus the potential role of vitamin D(3) in skeletal muscle. In this study, we isolated and sequenced the full-length Vdr and Cyp27b1 transcripts in C2C12 myoblasts and myotubes. Western blots and immunocytochemistry confirmed protein expression in both myoblasts and myotubes clearly demonstrating that C2C12 cells express VDR and CYP27B1. To determine the vitamin D(3) action, we found that C2C12 myoblasts treated with either 1α,25(OH)(2)D(3) or 25(OH)D(3) inhibited cell proliferation and this was associated with increased Vdr expression. The observation that treatment of C2C12 myoblasts with the inactive form of vitamin D(3), [25(OH)D(3)], inhibited proliferation suggested that CYP27B1 was functionally active. We used small interfering RNA to knock down Cyp27b1 in myoblasts, and cells were treated with 25(OH)D(3). The growth-suppressive effects of 25(OH)D(3) were abolished, suggesting that CYP27B1 in myoblasts is necessary for the ability of 25(OH)D(3) to affect cell proliferation. Finally, we analyzed expression of VDR and CYP27B1 in regenerating skeletal muscle in vivo. We found that expression of VDR and CYP27B1 increased significantly at day 7 of regeneration, and these results confirm the expression of Vdr and Cyp27b1 in vivo and suggest a potential role for vitamin D(3) in skeletal muscle regeneration following injury.
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