ArticleAmerican journal of physiology. Cell physiology2012
Cyclic AMP-induced K+ secretion occurs independently of Cl- secretion in rat distal colon.
Article in American journal of physiology. Cell physiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 19 citations in OpenAlex.
- Somatostatin peptides prevent increased human colonic epithelial permeability induced by hypoxia.American journal of physiology. Gastrointestinal and liver physiology · 2024Article
- The LRRC family of BK channel regulatory subunits: potential roles in health and disease.The Journal of physiology · 2022Review
- Aldosterone up-regulates basolateral NaFASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2021Article
- Goblet cell LRRC26 regulates BK channel activation and protects against colitis in mice.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Parallel intermediate conductance KAmerican journal of physiology. Gastrointestinal and liver physiology · 2020Article
- Adenylyl cyclase 6 is involved in the hyposecretory status of experimental colitis.Pflugers Archiv : European journal of physiology · 2018Article
- Colonic Potassium Absorption and Secretion in Health and Disease.Comprehensive Physiology · 2018Review
- Dextran sulfate sodium-induced chronic colitis attenuates CaAmerican journal of physiology. Cell physiology · 2018Article
- Mechanistic insight into the heme-independent interplay between iron and carbon monoxide in CFTR and Slo1 BKMetallomics : integrated biometal science · 2017Review
- Segmental differences in upregulated apical potassium channels in mammalian colon during potassium adaptation.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
- Shikonin Inhibits Intestinal Calcium-Activated Chloride Channels and Prevents Rotaviral Diarrhea.Frontiers in pharmacology · 2016Article
- Na-H Exchanger Isoform-2 (NHE2) Mediates Butyrate-dependent Na+ Absorption in Dextran Sulfate Sodium (DSS)-induced Colitis.The Journal of biological chemistry · 2015Article
- A whole-genome RNAi screen uncovers a novel role for human potassium channels in cell killing by the parasite Entamoeba histolytica.Scientific reports · 2015Article
- Enhanced K(+) secretion in dextran sulfate-induced colitis reflects upregulation of large conductance apical K(+) channels (BK; Kcnma1).American journal of physiology. Cell physiology · 2013Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
Abstract
cAMP induces both active Cl(-) and active K(+) secretion in mammalian colon. It is generally assumed that a mechanism for K(+) exit is essential to maintain cells in the hyperpolarized state, thus favoring a sustained Cl(-) secretion. Both Kcnn4c and Kcnma1 channels are located in colon, and this study addressed the questions of whether Kcnn4c and/or Kcnma1 channels mediate cAMP-induced K(+) secretion and whether cAMP-induced K(+) secretion provides the driving force for Cl(-) secretion. Forskolin (FSK)-enhanced short-circuit current (indicator of net electrogenic ion transport) and K(+) fluxes were measured simultaneously in colonic mucosa under voltage-clamp conditions. Mucosal Na(+) orthovanadate (P-type ATPase inhibitor) inhibited active K(+) absorption normally present in rat distal colon. In the presence of mucosal Na(+) orthovanadate, serosal FSK induced both K(+) and Cl(-) secretion. FSK-induced K(+) secretion was 1) not inhibited by either mucosal or serosal 1-[(2-chlorophenyl) diphenylmethyl]-1H-pyrazole (TRAM-34; a Kcnn4 channel blocker), 2) inhibited (92%) by mucosal iberiotoxin (Kcnma1 channel blocker), and 3) not affected by mucosal cystic fibrosis transmembrane conductance regulator inhibitor (CFTR(inh)-172). By contrast, FSK-induced Cl(-) secretion was 1) completely inhibited by serosal TRAM-34, 2) not inhibited by either mucosal or serosal iberiotoxin, and 3) completely inhibited by mucosal CFTR(inh)-172. These results indicate that cAMP-induced colonic K(+) secretion is mediated via Kcnma1 channels located in the apical membrane and most likely contributes to stool K(+) losses in secretory diarrhea. On the other hand, cAMP-induced colonic Cl(-) secretion requires the activity of Kcnn4b channels located in the basolateral membrane and is not dependent on the concurrent activation of apical Kcnma1 channels.
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