ArticleMedical science monitor : international medical journal of experimental and clinical research2012
Improved real-time PCR assay for detection and quantification of all 54 known types of human adenoviruses in clinical samples.
Article in Medical science monitor : international medical journal of experimental and clinical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 14 citations in OpenAlex.
- Mitigating Future Respiratory Virus Pandemics: New Threats and Approaches to Consider.Viruses · 2021Review
- Cytokine/Chemokine Expression Is Closely Associated Disease Severity of Human Adenovirus Infections in Immunocompetent Adults and Predicts Disease Progression.Frontiers in immunology · 2021Article
- Virus detections among patients with severe acute respiratory illness, Northern Vietnam.PloS one · 2020Article
- Bioaerosol Sampling for Respiratory Viruses in Singapore's Mass Rapid Transit Network.Scientific reports · 2018Article
- Co-infection with human polyomavirus BK enhances gene expression and replication of human adenovirus.Archives of virology · 2018Article
- Epidemiological study of people living in rural North Carolina for novel respiratory viruses.Zoonoses and public health · 2018Article
- Surveillance for respiratory and diarrheal pathogens at the human-pig interface in Sarawak, Malaysia.PloS one · 2018Article
- Aerosol Sampling in a Hospital Emergency Room Setting: A Complementary Surveillance Method for the Detection of Respiratory Viruses.Frontiers in public health · 2018Article
- Molecular surveillance of respiratory viruses with bioaerosol sampling in an airport.Tropical diseases, travel medicine and vaccines · 2018Article
- Bioaerosol Sampling in Clinical Settings: A Promising, Noninvasive Approach for Detecting Respiratory Viruses.Open forum infectious diseases · 2017Article
- Epidemiology of human adenovirus and molecular characterization of human adenovirus 55 in China, 2009-2012.Influenza and other respiratory viruses · 2014Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDetection and quantification of adenoviruses (AdVs) causing life-threatening complications are important abilities in recognition of infection and management of immunocompromised patients. Due to the rapid increase in the number of known AdV types, most commercial tests for detection and identification of AdVs are outdated. MATERIAL/
methodsWe designed an improved, easier and faster real-time quantitative polymerase chain reaction (RQ-PCR) method for detection and quantification of 54 types of human AdVs. A wide validation effort was undertaken to ensure confidence in highly sensitive and specific detection of AdVs in compromised patients. The validation process included evaluation of the method's suitability and reliability for use in routine diagnostics.
resultsDue to high sensitivity (9.2×10² copies/ml) and broad dynamic range (7 log) we are able to detect specific viral DNA in large amounts of cell-free body fluids. The new assay is characterized by high precision and low variation within and between individual virus tests (CV=0.036%, CV=1.29%), low bias error (4%) and no cross-reactivity with other pathogens.
conclusionsThe implementation of this new assay in clinical and laboratory practice provides a rapid, reliable and less laborious method for detection and monitoring of AdV replication in immunocompromised patients. Moreover, it offers the ability to distinguish between active and latent infection and assess treatment efficiency.
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