ArticleProceedings of the National Academy of Sciences of the United States of America2012
Strategy for enhanced transgenic strain development for embryonic conditional lethality in Anastrepha suspensa.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 89 citations in OpenAlex.
- Neoclassical development of genetic sexing strains for insect pest and disease vector control.Insect science · 2026Review
- Chromosome-scale genome assembly of the South American fruit fly, Anastrepha fraterculus sp.1.Insect science · 2026Article
- Sequence and expression analysis of potential spermatogenesis-specific gene cognates in the Caribbean fruit fly, Anastrepha suspensa.Insect science · 2026Article
- Genetic engineering for SIT application: a fruit fly-focused review.Insect science · 2026Review
- Stage-resolved transcriptomic profiling ofFrontiers in insect science · 2025Article
- ImprovedInternational journal of molecular sciences · 2023Article
- Sequence and expression analysis of the spermatogenesis-specific gene cognates, wampa and Prosα6T, in Drosophila suzukii.Genetica · 2023Article
- Effects of antibiotics on theFrontiers in bioengineering and biotechnology · 2023Article
- Highly Efficient Temperature Inducible CRISPR-Cas9 Gene Targeting inInternational journal of molecular sciences · 2021Article
- A transgenic female killing system for the genetic control of Drosophila suzukii.Scientific reports · 2021Article
- Transcriptome analysis of Anastrepha fraterculus sp. 1 males, females, and embryos: insights into development, courtship, and reproduction.BMC genetics · 2020Article
- Identification and characterization of four Drosophila suzukii cellularization genes and their promoters.BMC genetics · 2020Article
- Genetic breakdown of a Tet-off conditional lethality system for insect population control.Nature communications · 2020Article
- Transcriptome Analysis of the Oriental Fruit FlyInsects · 2020Article
- Improved transgenic sexing strains for genetic control of the Australian sheep blow fly Lucilia cuprina using embryo-specific gene promoters.Molecular genetics and genomics : MGG · 2020Article
- Overexpression of an antioxidant enzyme improves male mating performance after stress in a lek-mating fruit fly.Proceedings. Biological sciences · 2019Article
- Molecular tools to create new strains for mosquito sexing and vector control.Parasites & vectors · 2018Review
- Transcriptional effects of a positive feedback circuit in Drosophila melanogaster.BMC genomics · 2017Article
- Temperature-dependent sex-reversal by a transformer-2 gene-edited mutation in the spotted wing drosophila, Drosophila suzukii.Scientific reports · 2017Article
- A Chromosome-Scale Assembly of theG3 (Bethesda, Md.) · 2017Article
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here the first reproductive sterility system for the tephritid fruit fly pest, Anastrepha suspensa, is presented, based on lethality primarily limited to embryos heterozygous for a conditional lethal transgene combination. This tetracycline (Tet)-suppressible system uses a driver construct having the promoter from the newly isolated embryo-specific A. suspensa serendipity α gene linked to the Tet-transactivator. This was used to drive expression of a phosphomutated variant of the pro-apoptotic cell death gene, hid, from A. ludens, that was isolated, based on its identity to A. suspensa hid. The Alhid(Ala2) variant was shown to have the highest cell death activity in an in vitro A. suspensa cell death assay compared to the orthologous genes Ashid, Dmhid, and the variant Dmhid(Ala5). These cell death assays also allowed a determination of the most-efficient driver-effector cassette combinations for use in A. suspensa transformants, resulting in two hybrid strains exhibiting 100% lethality. One strain was 96% lethal in embryos in the absence of tetracycline, with none surviving past the first larval instar, which is critical for pests that are most damaging in late-larval stages. We demonstrate that the isolation and in vitro validation of species-specific promoters and lethal effector genes can greatly improve the efficiency of creating high-performance conditional lethality strains that may be extended to other insect pest species.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.