ArticleBMC microbiology2012
Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress.
Article in BMC microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Revealing potential functions of hypothetical proteins induced by genistein in the symbiosis island of Bradyrhizobium japonicum commercial strain SEMIA 5079 (= CPAC 15).BMC microbiology · 2022Article
- Alterations of Primary Metabolites in Root Exudates of Intercropped Cajanus cajan-Zea mays Modulate the Adaptation and Proteome of Ensifer (Sinorhizobium) fredii NGR234.Microbial ecology · 2022Article
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- Transcriptomic Studies Reveal that theInternational journal of molecular sciences · 2019Article
- Natural rhizobial diversity helps to reveal genes and QTLs associated with biological nitrogen fixation in common bean.AIMS microbiology · 2017Review
- Genome of Rhizobium leucaenae strains CFN 299(T) and CPAO 29.8: searching for genes related to a successful symbiotic performance under stressful conditions.BMC genomics · 2016Article
- RNA-seq analysis of the Rhizobium tropici CIAT 899 transcriptome shows similarities in the activation patterns of symbiotic genes in the presence of apigenin and salt.BMC genomics · 2016Article
- Proteomic analysis of Chromobacterium violaceum and its adaptability to stress.BMC microbiology · 2015Article
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- Nodulation Characterization and Proteomic Profiling of Bradyrhizobium liaoningense CCBAU05525 in Response to Water-Soluble Humic Materials.Scientific reports · 2015Article
- RNA-Seq analysis of the multipartite genome of Rhizobium etli CE3 shows different replicon contributions under heat and saline shock.BMC genomics · 2014Article
- Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis.BMC genomics · 2014Article
- Global transcriptional response to heat shock of the legume symbiont Mesorhizobium loti MAFF303099 comprises extensive gene downregulation.DNA research : an international journal for rapid publication of reports on genes and genomes · 2014Article
- Effect of leguminous lectins on the growth of Rhizobium tropici CIAT899.Molecules (Basel, Switzerland) · 2013Article
- Article
- Characterization of rhizobia isolates obtained from nodules of wild genotypes of common bean.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRhizobium tropici strain PRF 81 (= SEMIA 4080) has been used in commercial inoculants for application to common-bean crops in Brazil since 1998, due to its high efficiency in fixing nitrogen, competitiveness against indigenous rhizobial populations and capacity to adapt to stressful tropical conditions, representing a key alternative to application of N-fertilizers. The objective of our study was to obtain an overview of adaptive responses to heat stress of strain PRF 81, by analyzing differentially expressed proteins when the bacterium is grown at 28°C and 35°C.
resultsTwo-dimensional gel electrophoresis (2DE) revealed up-regulation of fifty-nine spots that were identified by MALDI-TOF/TOF-TOF. Differentially expressed proteins were associated with the functional COG categories of metabolism, cellular processes and signaling, information storage and processing. Among the up-regulated proteins, we found some related to conserved heat responses, such as molecular chaperones DnaK and GroEL, and other related proteins, such as translation factors EF-Tu, EF-G, EF-Ts and IF2. Interestingly, several oxidative stress-responsive proteins were also up-regulated, and these results reveal the diversity of adaptation mechanisms presented by this thermotolerant strain, suggesting a cross-talk between heat and oxidative stresses.
conclusionsOur data provide valuable protein-expression information relevant to the ongoing genome sequencing of strain PRF 81, and contributes to our still-poor knowledge of the molecular determinants of the thermotolerance exhibited by R. tropici species.
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