ArticleExpert opinion on drug discovery2011
Lessons from high-throughput protein crystallization screening: 10 years of practical experience.
Article in Expert opinion on drug discovery, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed.
- LEGO®-inspired electrically-actuated microfluidics for on-chip protein crystallization andLab on a chip · 2026Article
- A bipartite bacterial virulence factor targets the complement system and neutrophil activation.The EMBO journal · 2025Article
- Scalable fabrication of an array-type fixed-target device for automated room temperature X-ray protein crystallography.Scientific reports · 2025Article
- Systematic enhancement of protein crystallization efficiency by bulk lysine-to-arginine (KR) substitution.Protein science : a publication of the Protein Society · 2024Article
- The NS1 protein of influenza B virus binds 5'-triphosphorylated dsRNA to suppress RIG-I activation and the host antiviral response.bioRxiv : the preprint server for biology · 2024Article
- Polymer-based microfluidic device for on-chip counter-diffusive crystallization andLab on a chip · 2023Article
- High-Throughput Screening to Obtain Crystal Hits for Protein Crystallography.Journal of visualized experiments : JoVE · 2023Article
- 20 years of crystal hits: progress and promise in ultrahigh-throughput crystallization screening.Acta crystallographica. Section D, Structural biology · 2023Article
- Article
- High-Throughput Crystallization Pipeline at the Crystallography Core Facility of the Institut Pasteur.Molecules (Basel, Switzerland) · 2019Review
- A restriction-free method for gene reconstitution using two single-primer PCRs in parallel to generate compatible cohesive ends.BMC biotechnology · 2017Article
- The use of haptic interfaces and web services in crystallography: an application for a 'screen to beam' interface.Journal of applied crystallography · 2016Article
- Crystal structure of rofecoxib bound to human cyclooxygenase-2.Acta crystallographica. Section F, Structural biology communications · 2016Article
- Substrate-selective Inhibition of Cyclooxygeanse-2 by Fenamic Acid Derivatives Is Dependent on Peroxide Tone.The Journal of biological chemistry · 2016Article
- Minimal physical requirements for crystal growth self-poisoning.The Journal of chemical physics · 2016Article
- The Crystal Structure of an Integral Membrane Fatty Acid α-Hydroxylase.The Journal of biological chemistry · 2015Article
- Structural characterization and modeling of the Borrelia burgdorferi hybrid histidine kinase Hk1 periplasmic sensor: A system for sensing small molecules associated with tick feeding.Journal of structural biology · 2015Article
- The detection and subsequent volume optimization of biological nanocrystals.Structural dynamics (Melville, N.Y.) · 2015Article
- A simple technique to reduce evaporation of crystallization droplets by using plate lids with apertures for adding liquids.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Identifying, studying and making good use of macromolecular crystals.Acta crystallographica. Section F, Structural biology communications · 2014Article
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
introductionX-ray crystallography provides the majority of our structural biological knowledge at a molecular level and, in terms of pharmaceutical design, is a valuable tool to accelerate discovery. It is the premier technique in the field, but its usefulness is significantly limited by the need to grow well-diffracting crystals. It is for this reason that high-throughput crystallization has become a key technology that has matured over the past 10 years through the field of structural genomics. Areas covered : The authors describe their experiences in high-throughput crystallization screening in the context of structural genomics and the general biomedical community. They focus on the lessons learnt from the operation of a high-throughput crystallization-screening laboratory, which to date has screened over 12,500 biological macromolecules. They also describe the approaches taken to maximize the success while minimizing the effort. Through this, the authors hope that the reader will gain an insight into the efficient design of a laboratory and protocols to accomplish high-throughput crystallization on a single-, multiuser laboratory or industrial scale. Expert opinion : High-throughput crystallization screening is readily available but, despite the power of the crystallographic technique, getting crystals is still not a solved problem. High-throughput approaches can help when used skillfully; however, they still require human input in the detailed analysis and interpretation of results to be more successful.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.