ArticleOpen biology2012
Spatiotemporal dynamics of Spc105 regulates the assembly of the Drosophila kinetochore.
Article in Open biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 35 citations in OpenAlex.
- Unraveling the kinetochore nanostructure in Schizosaccharomyces pombe using multi-color SMLM imaging.The Journal of cell biology · 2023Article
- Shaping centromeres to resist mitotic spindle forces.Journal of cell science · 2022Article
- Kinetochore Architecture Employs Diverse Linker Strategies Across Evolution.Frontiers in cell and developmental biology · 2022Review
- The microtubule- and PP1-binding activities ofMolecular biology of the cell · 2022Article
- Borealin directs recruitment of the CPC to oocyte chromosomes and movement to the microtubules.The Journal of cell biology · 2021Article
- CENP-C functions in centromere assembly, the maintenance of CENP-A asymmetry and epigenetic age in Drosophila germline stem cells.PLoS genetics · 2021Article
- Bridgin connects the outer kinetochore to centromeric chromatin.Nature communications · 2021Article
- The whole is greater than the sum of its parts: at the intersection of order, disorder, and kinetochore function.Essays in biochemistry · 2020Review
- The regulation of chromosome segregation via centromere loops.Critical reviews in biochemistry and molecular biology · 2019Review
- Kinetochore Proteins Have a Post-Mitotic Function in Neurodevelopment.Developmental cell · 2019Article
- The Kinetochore Protein Spc105, a Novel Interaction Partner of LaeA, Regulates Development and Secondary Metabolism inFrontiers in microbiology · 2019Article
- Creation and testing of a new, local microtubule-disruption tool based on the microtubule-severing enzyme, katanin p60.Cytoskeleton (Hoboken, N.J.) · 2018Article
- Drosophila Nnf1 paralogs are partially redundant for somatic and germ line kinetochore function.Chromosoma · 2017Article
- A Molecular View of Kinetochore Assembly and Function.Biology · 2017Review
- An essential step of kinetochore formation controlled by the SNARE protein Snap29.The EMBO journal · 2016Article
- Getting to the heart of an unusual kinetochore.Open biology · 2016Article
- Network of protein interactions within the Drosophila inner kinetochore.Open biology · 2016Article
- Insights from the reconstitution of the divergent outer kinetochore of Drosophila melanogaster.Open biology · 2016Article
- CENP-C and CENP-I are key connecting factors for kinetochore and CENP-A assembly.Journal of cell science · 2015Article
- Repetitive centromeric satellite RNA is essential for kinetochore formation and cell division.The Journal of cell biology · 2014Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
The formation of kinetochores shortly before each cell division is a prerequisite for proper chromosome segregation. The synchronous mitoses of Drosophila syncytial embryos have provided an ideal in vivo system to follow kinetochore assembly kinetics and so address the question of how kinetochore formation is regulated. We found that the nuclear exclusion of the Spc105/KNL1 protein during interphase prevents precocious assembly of the Mis12 complex. The nuclear import of Spc105 in early prophase and its immediate association with the Mis12 complex on centromeres are thus the first steps in kinetochore assembly. The cumulative kinetochore levels of Spc105 and Mis12 complex then determine the rate of Ndc80 complex recruitment commencing only after nuclear envelope breakdown. The carboxy-terminal part of Spc105 directs its nuclear import and is sufficient for the assembly of all core kinetochore components and CENP-C, when localized ectopically to centrosomes. Super-resolution microscopy shows that carboxy-terminus of Spc105 lies at the junction of the Mis12 and Ndc80 complexes on stretched kinetochores. Our study thus indicates that physical accessibility of kinetochore components plays a crucial role in the regulation of Drosophila kinetochore assembly and leads us to a model in which Spc105 is a licensing factor for its onset.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.