ArticleOpen biology2012
Structure and function of the transketolase from Mycobacterium tuberculosis and comparison with the human enzyme.
Article in Open biology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 47 citations in OpenAlex.
- CRISPRi knockdown of mycobacterial tkt gene potentiates the anti-mycobacterial activity of phyto-compounds from selected medicinal plants.BMC complementary medicine and therapies · 2025Article
- Effect of xylitol on low‑density lipoprotein‑stimulated oxidative stress in THP‑1 cells.Molecular medicine reports · 2025Article
- Understanding current challenges of antibiotic resistance to find new solutions for 2050: scientific, medical and political issues.Archives of microbiology · 2025Review
- Comparative transcriptomic signatures of virulent and attenuatedFrontiers in cellular and infection microbiology · 2025Article
- Structural determination and kinetic analysis of the transketolase from Vibrio vulnificus reveal unexpected cooperative behavior.Protein science : a publication of the Protein Society · 2024Article
- Discovery of Novel Transketolase Epitopes and the Development of IgG-Based Tuberculosis Serodiagnostics.Microbiology spectrum · 2023Article
- Characterisation of a hyperthermophilic transketolase fromOrganic & biomolecular chemistry · 2021Article
- A 'Split-Gene' Transketolase From the Hyper-Thermophilic BacteriumFrontiers in microbiology · 2020Article
- d-Ribose as a Contributor to Glycated Haemoglobin.EBioMedicine · 2017Article
- Novel European free-living, non-diazotrophic Bradyrhizobium isolates from contrasting soils that lack nodulation and nitrogen fixation genes - a genome comparison.Scientific reports · 2016Article
- High-resolution structures of Lactobacillus salivarius transketolase in the presence and absence of thiamine pyrophosphate.Acta crystallographica. Section F, Structural biology communications · 2015Article
- Cloning, expression and characterization of sugarcane (Saccharum officinarum L.) transketolase.The protein journal · 2013Article
- Article
- Isolation and Biochemical Characterization of Recombinant Transketolase from Mycobacterium tuberculosis.Acta naturaeArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transketolase (TKT) enzyme in Mycobacterium tuberculosis represents a novel drug target for tuberculosis treatment and has low homology with the orthologous human enzyme. Here, we report on the structural and kinetic characterization of the transketolase from M. tuberculosis (TBTKT), a homodimer whose monomers each comprise 700 amino acids. We show that TBTKT catalyses the oxidation of donor sugars xylulose-5-phosphate and fructose-6-phosphate as well as the reduction of the acceptor sugar ribose-5-phosphate. An invariant residue of the TKT consensus sequence required for thiamine cofactor binding is mutated in TBTKT; yet its catalytic activities are unaffected, and the 2.5 Å resolution structure of full-length TBTKT provides an explanation for this. Key structural differences between the human and mycobacterial TKT enzymes that impact both substrate and cofactor recognition and binding were uncovered. These changes explain the kinetic differences between TBTKT and its human counterpart, and their differential inhibition by small molecules. The availability of a detailed structural model of TBTKT will enable differences between human and M. tuberculosis TKT structures to be exploited to design selective inhibitors with potential antitubercular activity.
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