ArticleFrontiers in plant science2011
Molecular screening tools to study Arabidopsis transcription factors.
Article in Frontiers in plant science, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- CmPlant direct · 2025Article
- Improving Drought Stress Tolerance in Ramie (Frontiers in plant science · 2022Review
- Identification of transcription factors interacting with a 1274 bp promoter of MaPIP1;1 which confers high-level gene expression and drought stress Inducibility in transgenic Arabidopsis thaliana.BMC plant biology · 2020Article
- NINJA-associated ERF19 negatively regulates Arabidopsis pattern-triggered immunity.Journal of experimental botany · 2019Article
- WRKY43 regulates polyunsaturated fatty acid content and seed germination under unfavourable growth conditions.Scientific reports · 2017Article
- Article
- Identification of Two bZIP Transcription Factors Interacting with the Promoter of Soybean Rubisco Activase Gene (GmRCAα).Frontiers in plant science · 2016Article
- Screen Identifying Arabidopsis Transcription Factors Involved in the Response to 9-Lipoxygenase-Derived Oxylipins.PloS one · 2016Article
- A genome-scale resource for the functional characterization of Arabidopsis transcription factors.Cell reports · 2014Article
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Authors and funding
3 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
In the model plant Arabidopsis thaliana, more than 2000 genes are estimated to encode transcription factors (TFs), which clearly emphasizes the importance of transcriptional control. Although genomic approaches have generated large TF open reading frame (ORF) collections, only a limited number of these genes is functionally characterized, yet. This review evaluates strategies and methods to identify TF functions. In particular, we focus on two recently developed TF screening platforms, which make use of publically available GATEWAY(®)-compatible ORF collections. (1) The Arabidopsis thalianaTF ORF over-Expression (AtTORF-Ex) library provides pooled collections of transgenic lines over-expressing HA-tagged TF genes, which are suited for screening approaches to define TF functions in stress defense and development. (2) A high-throughput microtiter plate based protoplast transactivation (PTA) system has been established to screen for TFs which are regulating a given promoter:Luciferase construct in planta.
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