ArticleFrontiers in endocrinology2011
THP-1 Macrophages and SGBS Adipocytes - A New Human in vitro Model System of Inflamed Adipose Tissue.
Article in Frontiers in endocrinology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Animal-Free Setup of a 3D Mature Adipocyte-Macrophage Co-Culture to Induce Inflammation In Vitro.Advanced healthcare materials · 2025Article
- Shielding Human Adipocytes From Inflammation: The Protective Potential of Polyphenol-Rich Opuntia ficus-indica Cladode Extract.Molecular nutrition & food research · 2025Article
- Trauma-associated extracellular histones mediate inflammation via a MYD88-IRAK1-ERK signaling axis and induce lytic cell death in human adipocytes.Cell death & disease · 2024Article
- Experimental cell models of insulin resistance: overview and appraisal.Frontiers in endocrinology · 2024Review
- Multi-omic integration reveals cell-type-specific regulatory networks of insulin resistance in distinct ancestry populations.Cell systems · 2023Article
- 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biology.International journal of obesity (2005) · 2022Review
- A new human adipocyte model with PTEN haploinsufficiency.Adipocyte · 2020Article
- iTRAQ-Based Quantitative Proteomic Comparison of 2D and 3D Adipocyte Cell Models Co-cultured with Macrophages Using Online 2D-nanoLC-ESI-MS/MS.Scientific reports · 2019Article
- On the role of macrophages in the control of adipocyte energy metabolism.Endocrine connections · 2019Review
- Antioxidant and Anti-Inflammatory Properties ofAntioxidants (Basel, Switzerland) · 2019Article
- Effects of exosomes from LPS-activated macrophages on adipocyte gene expression, differentiation, and insulin-dependent glucose uptake.Journal of physiology and biochemistry · 2018Article
- Activated macrophages control human adipocyte mitochondrial bioenergetics via secreted factors.Molecular metabolism · 2017Article
- miR-146a-mediated suppression of the inflammatory response in human adipocytes.Scientific reports · 2016Article
- Cell Models and Their Application for Studying Adipogenic Differentiation in Relation to Obesity: A Review.International journal of molecular sciences · 2016Review
- Interaction of differentiated human adipocytes with macrophages leads to trogocytosis and selective IL-6 secretion.Cell death & disease · 2015Article
- Obesity and inflammation: reduced cytokine expression due to resveratrol in a human in vitro model of inflamed adipose tissue.Frontiers in pharmacology · 2015Article
- Resveratrol suppresses PAI-1 gene expression in a human in vitro model of inflamed adipose tissue.Oxidative medicine and cellular longevity · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Obesity is associated with an accumulation of macrophages in adipose tissue. This inflammation of adipose tissue is a key event in the pathogenesis of several obesity-related disorders, particularly insulin resistance. Here, we summarized existing model systems that mimic the situation of inflamed adipose tissue in vitro, most of them being murine. Importantly, we introduce our newly established human model system which combines the THP-1 monocytic cell line and the preadipocyte cell strain Simpson-Golabi-Behmel syndrome (SGBS). THP-1 cells, which originate from an acute monocytic leukemia, differentiate easily into macrophages in vitro. The human preadipocyte cell strain SGBS was recently introduced as a unique tool to study human fat cell functions. SGBS cells are characterized by a high capacity for adipogenic differentiation. SGBS adipocytes are capable of fat cell-specific metabolic functions such as insulin-stimulated glucose uptake, insulin-stimulated de novo lipogenesis and β-adrenergic-stimulated lipolysis and they secrete typical adipokines including leptin, adiponectin, and RBP4. Applying either macrophage-conditioned medium or a direct co-culture of macrophages and fat cells, our model system can be used to distinguish between paracrine and cell-contact dependent effects. In conclusion, we propose this model as a useful tool to study adipose inflammation in vitro. It represents an inexpensive, highly reproducible human system. The methods described here can be easily extended for usage of primary human macrophages and fat cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.