ArticleArchaea (Vancouver, B.C.)2012
Archaeal phospholipid biosynthetic pathway reconstructed in Escherichia coli.
Article in Archaea (Vancouver, B.C.), 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 23 citations in OpenAlex.
- Article
- Optimizing Archaeal Lipid Biosynthesis inACS synthetic biology · 2024Article
- Bridging the membrane lipid divide: bacteria of the FCB group superphylum have the potential to synthesize archaeal ether lipids.The ISME journal · 2021Article
- Construction of an artificial biosynthetic pathway for hyperextended archaeal membrane lipids in the bacteriumSynthetic biology (Oxford, England) · 2020Article
- Isoprenoids enhance the stability of fatty acid membranes at the emergence of life potentially leading to an early lipid divide.Interface focus · 2019Article
- ConvertingProceedings of the National Academy of Sciences of the United States of America · 2018Article
- Geranylgeranyl reductase and ferredoxin from Methanosarcina acetivorans are required for the synthesis of fully reduced archaeal membrane lipid in Escherichia coli cells.Journal of bacteriology · 2014Article
- Biosynthesis of archaeal membrane ether lipids.Frontiers in microbiology · 2014Review
- A versatile cis-prenyltransferase from Methanosarcina mazei catalyzes both C- and O-prenylations.The Journal of biological chemistryArticle
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A part of the biosynthetic pathway of archaeal membrane lipids, comprised of 4 archaeal enzymes, was reconstructed in the cells of Escherichia coli. The genes of the enzymes were cloned from a mesophilic methanogen, Methanosarcina acetivorans, and the activity of each enzyme was confirmed using recombinant proteins. In vitro radioassay showed that the 4 enzymes are sufficient to synthesize an intermediate of archaeal membrane lipid biosynthesis, that is, 2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphate, from precursors that can be produced endogenously in E. coli. Introduction of the 4 genes into E. coli resulted in the production of archaeal-type lipids. Detailed liquid chromatography/electron spray ionization-mass spectrometry analyses showed that they are metabolites from the expected intermediate, that is, 2,3-di-O-geranylgeranyl-sn-glycerol and 2,3-di-O-geranylgeranyl-sn-glycerol-1-phosphoglycerol. The metabolic processes, that is, dephosphorylation and glycerol modification, are likely catalyzed by endogenous enzymes of E. coli.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.