ArticleProceedings of the National Academy of Sciences of the United States of America2012
Domain activities of PapC usher reveal the mechanism of action of an Escherichia coli molecular machine.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed.
- Comparative in vitro activity of furazidin and nitrofurantoin and assessment of resistance mechanisms in Escherichia coli isolates from urinary tract infections.Folia microbiologica · 2026Article
- Gating of β-Barrel Protein Pores, Porins, and Channels: An Old Problem with New Facets.International journal of molecular sciences · 2023Review
- FimH and Type 1 Pili Mediated Tumor Cell Cytotoxicity by UropathogenicPathogens (Basel, Switzerland) · 2023Article
- Catheter-Associated Urinary Tract Infections: Current Challenges and Future Prospects.Research and reports in urology · 2022Review
- Processive dynamics of the usher assembly platform during uropathogenic Escherichia coli P pilus biogenesis.Nature communications · 2021Article
- Fimbriae reprogram host gene expression - Divergent effects of P and type 1 fimbriae.PLoS pathogens · 2019Article
- Reaching the End of the Line: Urinary Tract Infections.Microbiology spectrum · 2019Review
- Therapeutic Approaches Targeting the Assembly and Function of Chaperone-Usher Pili.EcoSal Plus · 2019Review
- Structural basis for usher activation and intramolecular subunit transfer in P pilus biogenesis in Escherichia coli.Nature microbiology · 2018Article
- Article
- Pili Assembled by the Chaperone/Usher Pathway inEcoSal Plus · 2018Review
- Drug and Vaccine Development for the Treatment and Prevention of Urinary Tract Infections.Microbiology spectrum · 2016Review
- Global redesign of a native β-barrel scaffold.Biochimica et biophysica acta · 2016Article
- Analysis of yeh Fimbrial Gene Cluster in Escherichia coli O157:H7 in Order to Find a Genetic Marker for this Serotype.Current microbiology · 2015Article
- The pilus usher controls protein interactions via domain masking and is functional as an oligomer.Nature structural & molecular biology · 2015Article
- Human Urine Decreases Function and Expression of Type 1 Pili in Uropathogenic Escherichia coli.mBio · 2015Article
- Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains.The EMBO journal · 2014Article
- Molecular basis of usher pore gating in Escherichia coli pilus biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Positively selected FimH residues enhance virulence during urinary tract infection by altering FimH conformation.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Ordered and ushered; the assembly and translocation of the adhesive type I and p pili.Biology · 2013Article
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Authors and funding
8 authors.
Funding
Abstract
P pili are prototypical chaperone-usher pathway-assembled pili used by Gram-negative bacteria to adhere to host tissues. The PapC usher contains five functional domains: a transmembrane β-barrel, a β-sandwich Plug, an N-terminal (periplasmic) domain (NTD), and two C-terminal (periplasmic) domains, CTD1 and CTD2. Here, we delineated usher domain interactions between themselves and with chaperone-subunit complexes and showed that overexpression of individual usher domains inhibits pilus assembly. Prior work revealed that the Plug domain occludes the pore of the transmembrane domain of a solitary usher, but the chaperone-adhesin-bound usher has its Plug displaced from the pore, adjacent to the NTD. We demonstrate an interaction between the NTD and Plug domains that suggests a biophysical basis for usher gating. Furthermore, we found that the NTD exhibits high-affinity binding to the chaperone-adhesin (PapDG) complex and low-affinity binding to the major tip subunit PapE (PapDE). We also demonstrate that CTD2 binds with lower affinity to all tested chaperone-subunit complexes except for the chaperone-terminator subunit (PapDH) and has a catalytic role in dissociating the NTD-PapDG complex, suggesting an interplay between recruitment to the NTD and transfer to CTD2 during pilus initiation. The Plug domain and the NTD-Plug complex bound all of the chaperone-subunit complexes tested including PapDH, suggesting that the Plug actively recruits chaperone-subunit complexes to the usher and is the sole recruiter of PapDH. Overall, our studies reveal the cooperative, active roles played by periplasmic domains of the usher to initiate, grow, and terminate a prototypical chaperone-usher pathway pilus.
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