ArticleProceedings of the National Academy of Sciences of the United States of America2012
Sex-lethal enables germline stem cell differentiation by down-regulating Nanos protein levels during Drosophila oogenesis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 51 papers.
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51 citing papers in PubMed, 88 citations in OpenAlex.
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- Genetic and Epigenetic Regulation ofJournal of developmental biology · 2023Review
- Enrichment of Undifferentiated Germline and Somatic Cells from Drosophila Testes.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Targeting Endogenous Loci That Function in Drosophila Germline Stem Cells.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Safeguarding Drosophila female germ cell identity depends on an H3K9me3 mini domain guided by a ZAD zinc finger protein.PLoS genetics · 2022Article
- Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26.Life science alliance · 2022Article
- Isolation and Characterization of Germline Stem Cells in Protogynous HermaphroditicInternational journal of molecular sciences · 2022Article
- Noncanonical function of the Sex lethal gene controls the protogyny phenotype in Drosophila melanogaster.Scientific reports · 2022Article
- The Regulation of Germline Sex Determination in Drosophila by Sex lethal.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2022Review
- Molecular population genetics of Sex-lethal (Sxl) in the Drosophila melanogaster species group: a locus that genetically interacts with Wolbachia pipientis in Drosophila melanogaster.G3 (Bethesda, Md.) · 2021Article
- Interplay between sex determination cascade and major signaling pathways during Drosophila eye development: Perspectives for future research.Developmental biology · 2021Review
- The Dynamic Regulation of mRNA Translation and Ribosome Biogenesis During Germ Cell Development and Reproductive Aging.Frontiers in cell and developmental biology · 2021Review
- Next-generation gene drive for population modification of the malaria vector mosquito,Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Molecular profiling of stem cell-like female germ line cells inBiology open · 2019Article
- Review
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3 authors at 1 institution in 1 country.
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Abstract
Drosophila ovarian germ cells require Sex-lethal (Sxl) to exit from the stem cell state and to enter the differentiation pathway. Sxl encodes a female-specific RNA binding protein and in somatic cells serves as the developmental switch gene for somatic sex determination and X-chromosome dosage compensation. None of the known Sxl target genes are required for germline differentiation, leaving open the question of how Sxl promotes the transition from stem cell to committed daughter cell. We address the mechanism by which Sxl regulates this transition through the identification of nanos as one of its target genes. Previous studies have shown that Nanos protein is necessary for GSC self-renewal and is rapidly down-regulated in the daughter cells fated to differentiate in the adult ovary. We find that this dynamic expression pattern is limited to female germ cells and is under Sxl control. In the absence of Sxl, or in male germ cells, Nanos protein is continuously expressed. Furthermore, this female-specific expression pattern is dependent on the presence of canonical Sxl binding sites located in the nanos 3' untranslated region. These results, combined with the observation that nanos RNA associates with the Sxl protein in ovarian extracts and loss and gain of function studies, suggest that Sxl enables the switch from germline stem cell to committed daughter cell by posttranscriptional down-regulation of nanos expression. These findings connect sexual identity to the stem cell self-renewal/differentiation decision and highlight the importance of posttranscriptional gene regulatory networks in controlling stem cell behavior.
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